Golestan University of Medical Sciences Repository

The effects of combined low level laser therapy and mesenchymal stem cells on bone regeneration in rabbit calvarial defects

Fekrazad, R. and Sadeghi Ghuchani, M. and Eslaminejad, M.B. and Taghiyar, L. and Kalhori, K.A.M. and Pedram, M.S. and Shayan, A.M. and Aghdami, N. and Abrahamse, H. (2015) The effects of combined low level laser therapy and mesenchymal stem cells on bone regeneration in rabbit calvarial defects. Journal of Photochemistry and Photobiology B: Biology, 151. pp. 180-185. ISSN 18732682

PDF - Published Version
Download (1MB) | Preview


Objective This study evaluated the effect of Low Level Laser Therapy (LLLT) and Mesenchymal Stem Cells (MSCs) on bone regeneration. Background data Although several studies evaluated the effects of MSCs and LLLT, there is little information available regarding in vivo application of LLLT in conjunction with MSCs. Methods Forty-eight circular bone defects (6 mm in diameter) were prepared in the calvaria of 12 New-Zealand white rabbits. The defects of each animal were randomly assigned to 4 groups: (C) no treatment; (L) applying LLLT; (SC) filled with MSCs; (SCL) application of both MSCs and LLLT. LLL was applied on alternate days at wavelength of 810 nm, power density of 0.2 W/cm2 and a fluency of 4 J/cm2 using a Gallium-Aluminum-Arsenide (GaAlAs) diode laser. The animals were sacrificed after 3 weeks and then histological samples were evaluated to determine the amount of new bone formation and the remaining scaffold and inflammation. Results The histological evaluation showed a statistically significant increase in new bone formation of LLLT group relative to the control and the other two experimental groups (p < 0.05). There was no significant difference in bone formation of the control group compared to experimental groups filled with MSCs. Laser irradiation had no significant effect on resorption of the scaffold material. In addition, inflammation was significantly reduced in LLLT group compared to the control defects and the other two experimental groups. Conclusion Low level laser therapy could be effective in bone regeneration but there is no evidence of a synergistic effect when applied in conjunction with MSCs. © 2015 Elsevier B.V. All rights reserved.

Item Type: Article
Additional Information: cited By 2
Uncontrolled Keywords: Animalia; Oryctolagus cuniculus
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 08 Sep 2016 17:26
Last Modified: 08 May 2018 07:28
URI: http://eprints.goums.ac.ir/id/eprint/4671

Actions (login required)

View Item View Item