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Effects of exercise training together with tamoxifen in reducing mammary tumor burden in mice: Possible underlying pathway of MIR-21

Khori, V. and Amani Shalamzari, S. and Isanejad, A. and Alizadeh, A.M. and Alizadeh, S. and Khodayari, S. and Khodayari, H. and Shahbazi, S. and Zahedi, A. and Sohanaki, H. and Khaniki, M. and Mahdian, R. and Saffari, M. and Fayad, R. (2015) Effects of exercise training together with tamoxifen in reducing mammary tumor burden in mice: Possible underlying pathway of MIR-21. European Journal of Pharmacology, 765. pp. 179-187.

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Abstract

Exercise training has an anti-tumor effect and can reduce tumor growth; however, the exact underlying mechanisms of its protective effects are still obscure. MicroRNA (miR)-21 is a predictor in cancer survival, and has a potential use as an indicator of therapeutic outcome in breast malignancies. Forty-eight female BALB/c mice were equally divided into six groups to investigate the effects of interval exercise training with tamoxifen on miR-21 expression and its possible assumed mechanisms in an estrogen receptor-positive breast cancer model. ELISA, immunohistochemistry, western blot, qRT-PCR assays were performed at the end of the study. Tumor size was significantly declined in exercise training and tamoxifen groups compared to tumor group (P<0.05). Expression of miR-21 was significantly down-regulated in trained and tamoxifen treated mice in comparison with tumor group (P<0.05). Exercise training was as effective as tamoxifen treatment in decreasing serum estradiol and ER-α expression (P<0.05). Exercise training and tamoxifen reduced tumor IL-6 levels, NF-kB and STAT3 expressions, and up-regulated TPM1 and PDCD4 expressions (P<0.05). Both exercise and tamoxifen had synergistic effects in reducing miR-21 and Bcl-2, and up-regulating PDCD4 expression. Results showed that interval exercise training may reduce mammary tumor burden in mice through possible underlying pathway of miR-21. © 2015 Elsevier B.V. All rights reserved.

Item Type: Article
Additional Information: cited By 4
Uncontrolled Keywords: alpha tropomyosin; estradiol; immunoglobulin enhancer binding protein; interleukin 6; microRNA 21; programmed death 1 receptor; protein bcl 2; STAT3 protein; tamoxifen; antineoplastic hormone agonists and antagonists; estradiol; interleukin 6; interleukin-6, mouse; microRNA; MIRN-21 microRNA, mouse; tamoxifen; tumor marker, animal cell; animal experiment; animal model; antineoplastic activity; Article; breast cancer; breast tumor; cancer survival; controlled study; down regulation; drug mechanism; enzyme linked immunosorbent assay; estradiol blood level; estrogen receptor positive breast cancer; exercise; female; immunohistochemistry; mouse; nonhuman; priority journal; protein expression; reverse transcription polymerase chain reaction; tumor growth; tumor volume; upregulation; Western blotting; animal; Bagg albino mouse; blood; drug effects; genetics; Mammary Neoplasms, Experimental; multimodality cancer therapy; pathology; physiology; tumor cell line, Animals; Antineoplastic Agents, Hormonal; Biomarkers, Tumor; Cell Line, Tumor; Combined Modality Therapy; Down-Regulation; Estradiol; Female; Interleukin-6; Mammary Neoplasms, Experimental; Mice, Inbred BALB C; MicroRNAs; Physical Conditioning, Animal; Tamoxifen; Tumor Burden
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 08 Sep 2016 09:48
Last Modified: 15 Jan 2017 17:14
URI: http://eprints.goums.ac.ir/id/eprint/4658

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