Golestan University of Medical Sciences Repository

Study of the association of mutant HBsAg gene and Hodgkin and non-Hodgkin lymphoma

Makvandi, K. and Ranjbari, N. and Makvandi, M. and Teimori, A.A. and Neisi, N. and Rasti, M. and Alipour, V. and Albokord, M. and Kanani, M. and Ahadi, R. and Habibian, A. (2015) Study of the association of mutant HBsAg gene and Hodgkin and non-Hodgkin lymphoma. Jundishapur Journal of Microbiology, 8 (11).

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Abstract

Background: Hepatitis B Virus (HBV) is responsible for chronic, acute, and fulminant hepatitis, which are prevalent worldwide. Chronic HBV may lead to cirrhosis and hepatocellular carcinoma. Several epidemiological studies have indicated that hepatitis B virus is involved in B-cell Hodgkin and Non-Hodgkin Lymphoma (NHL).Objectives: The aim of this study was to evaluate the association between hepatitis B infection and Hodgkin and non-Hodgkin Lymphoma.Materials and Methods: Paraffin embedded of 41 block samples including 12 (29.26%) Hodgkin and 29 (70.73%) non-Hodgkin patients were collected. Next, DNA extraction was carried out for all the samples followed by HBV DNA detection by the nested polymerase chain reaction (PCR). The positive HBV DNA samples were sequenced, and HBV genotypes and HBV subtypes were determined.Results: Three out of 12 (25%) Hodgkin samples and seven out of 29 (24.13%) non-Hodgkin showed positive HBV DNA results. The results of sequencing revealed that the D genotype was predominant among the positive HBV patients. Interestingly an unpredictable amino acid proline was detected in position 88 of the HBs gene, which indicates a new mutation in the “S” region of HBV DNA in patients with Hodgkin and non-Hodgkin lymphoma. Conclusions: A high rate of 25% and 24.13% of HBV DNA was detected among patients with Hodgkin and non-Hodgkin lymphoma, respectively. © 2015, Ahvaz Jundishapur University of Medical Sciences.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: amino acid; DNA; proline; virus DNA, acute hepatitis; adult; amino acid substitution; Article; clinical article; controlled study; disease association; DNA extraction; female; gene; gene mutation; gene sequence; genotype; HBsAg gene; hepatitis; hepatitis B; Hepatitis B virus; Hodgkin disease; human; male; nonhodgkin lymphoma; nucleotide sequence; nucleotide sequence; open reading frame; polymerase chain reaction; prevalence; sequence analysis
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 08 Sep 2016 09:34
Last Modified: 08 Sep 2016 09:34
URI: http://eprints.goums.ac.ir/id/eprint/4651

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