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Genetic variation in the ABCB1 gene may lead to mRNA level change: Application to gastric cancer cases

Mansoori, M. and Golalipour, M. and Alizadeh, S. and Jahangirerad, A. and Khandozi, S.R. and Fakharai, H. and Shahbazi, M. (2016) Genetic variation in the ABCB1 gene may lead to mRNA level change: Application to gastric cancer cases. Asian Pacific Journal of Cancer Prevention, 16 (18). pp. 8467-8471.

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Abstract

Background: One of the major mechanisms for drug resistance is associated with altered anticancer drug transport, mediated by the human-adenosine triphosphate binding cassette (ABC) transporter superfamily proteins. The overexpression of adenosine triphosphate binding cassette, sub-family B, member 1 (ABCB1) by multidrug-resistant cancer cells is a serious impediment to chemotherapy. In our study we have studied the possibility that structural single-nucleotide polymorphisms (SNP) are the mechanism of ABCB1 overexpression. Materials and Methods: A total of 101 gastric cancer multidrug resistant cases and 100 controls were genotyped with sequence-specific primed PCR (SSP-PCR). Gene expression was evaluated for 70 multidrug resistant cases and 54 controls by real time PCR. The correlation between the two groups was based on secondary structures of RNA predicted by bioinformatics tool. Results: The results of genotyping showed that among 3 studied SNPs, rs28381943 and rs2032586 had significant differences between patient and control groups but there were no differences in the two groups for C3435T. The results of real time PCR showed over-expression of ABCB1 when we compared our data with each of the genotypes in average mode. Prediction of secondary structures in the existence of 2 related SNPs (rs28381943 and rs2032586) showed that the amount of ΔG for original mRNA is higher than the amount of ΔG for the two mentioned SNPs. Conclusions: We have observed that 2 of our studied SNPs (rs283821943 and rs2032586) may elevate the expression of ABCB1 gene, through increase in mRNA stability, while this was not the case for C3435T.

Item Type: Article
Additional Information: cited By 2
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 08 Sep 2016 05:20
Last Modified: 27 Sep 2016 11:22
URI: http://eprints.goums.ac.ir/id/eprint/4609

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