Yazdani, Y. and Farazmandfar, T. and Azadeh, H. and Zekavatian, Z.
(2016)
The prognostic effect of PTEN expression status in colorectal cancer development and evaluation of factors affecting it: MiR-21 and promoter methylation.
Journal of Biomedical Science, 23 (1).
ISSN 14230127
Abstract
Background: PTEN is a tumor suppressor gene which is involved in cellular proliferation, differentiation, and apoptosis. Loss or down-regulation of PTEN plays an important role in human cancers development. In this study, we investigated the effect of miR-21 and promoter methylation on the PTEN expression status in CRC tissues and analyzed association of the PTEN expression status with clinicopathological features in patients with CRC. Results: The PTEN expression was positively detected in 67.2 CRC tissues and all adjacent non-cancerous samples. PTEN mRNA level was negatively correlated with miR-21 level (r = -0.595, P < 0.001). PTEN expression was also correlated directly with the PTEN mRNA level (r = 0.583, P < 0.001) and conversely with miR-21 level (r = -0.632, P < 0.001). PTEN Promoter methylation was significantly associated with PTEN expression status (p = 0.013). PTEN expression was negatively associated with tumor size (p = 0.007) and advanced tumor stage (P = 0.011). Multivariate analysis indicated that tumor stage, tumor differentiation and PTEN expression status were independent prognostic factors for overall carcinoma in CRC patients (P < 0.05). The Kaplan-Meier curve indicated a negative correlation between PTEN expression levels and survival of CRC patients (P = 0.013). Conclusions: This study suggests a high frequency of miR-21 overexpression and aberrant promoter methylation in down-regulation of PTEN expression in colorectal carcinoma. Loss of PTEN may be a prognostic factor for patients with CRC. © 2016 Yazdani et al.
Item Type: |
Article
|
Additional Information: |
cited By 1 |
Uncontrolled Keywords: |
microRNA 21; phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase, adult; advanced cancer; Article; cancer prognosis; cancer staging; cancer survival; cancer tissue; colorectal carcinoma; controlled study; disease association; DNA methylation; female; human; human tissue; male; middle aged; overall survival; priority journal; promoter region; protein expression; protein localization; tumor differentiation; tumor volume |
Subjects: |
مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus |
Divisions: |
معاونت تحقیقات و فناوری |
Depositing User: |
GOUMS
|
Date Deposited: |
08 Sep 2016 05:13 |
Last Modified: |
08 May 2018 04:50 |
URI: |
http://eprints.goums.ac.ir/id/eprint/4600 |
Actions (login required)
 |
View Item |