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Gonadectomy reduces the density of androgen receptor-immunoreactive neurons in male rat's hippocampus: Testosterone replacement compensates it

Moghadami, S. and Jahanshahi, M. and Sepehri, H. and Amini, H. (2016) Gonadectomy reduces the density of androgen receptor-immunoreactive neurons in male rat's hippocampus: Testosterone replacement compensates it. Behavioral and Brain Functions, 12 (1).

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Abstract

Background: In the present study, the role of gonadectomy on memory impairment and the density of androgen receptor-immunoreactive neurons in rats' hippocampus as well as the ability of testosterone to compensate of memory and the density of androgen receptors in the hippocampus was evaluated. Methods: Adult male rats (except intact-no testosterone group) were bilaterally castrated, and behavioral tests performed 2weeks later. Animals bilaterally cannulated into lateral ventricles and then received testosterone (10, 40 and 120μg/0.5μl DMSO) or vehicle (DMSO; 0.5μl) for gonadectomized-vehicle group, 30min before training in water maze test. The androgen receptor-immunoreactive neurons were detected by immunohistochemical technique in the hippocampal areas. Results: In the gonadectomized male rats, a memory deficit was found in Morris water maze test on test day (5th day) after DMSO administration. Gonadectomy decreased density of androgen receptor-immunoreactive neurons in the rats' hippocampus. The treatment with testosterone daily for 5days attenuated memory deficits induced by gonadectomy. Testosterone also significantly increased the density of androgen receptor-immunoreactive neurons in the hippocampal areas. The intermediate dose of this hormone (40μg) appeared to have a significant effect on spatial memory and the density of androgen receptor-immunoreactive neurons in gonadectomized rats' hippocampus. Conclusions: The present study suggests that testosterone can compensate memory failure in gonadectomized rats. Also testosterone replacement can compensate the reduction of androgen receptor-immunoreactive neurons density in the rats' hippocampus after gonadectomy. © 2016 Moghadami et al.

Item Type: Article
Additional Information: cited By 1
Uncontrolled Keywords: androgen receptor; testosterone, adult; androgen therapy; animal cell; animal experiment; animal model; animal tissue; Article; behavior assessment; brain lateral ventricle; cell density; controlled study; dentate gyrus; dose response; drug megadose; gonadectomy; hippocampal CA1 region; hippocampal CA3 region; hippocampus; immunohistochemistry; immunoreactivity; latent period; low drug dose; male; memory disorder; Morris water maze test; nonhuman; priority journal; rat; spatial memory
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 08 Sep 2016 05:11
Last Modified: 23 Jan 2017 10:46
URI: http://eprints.goums.ac.ir/id/eprint/4596

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