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Molecular and biochemical evidence on the protection of cardiomyocytes from phosphine-induced oxidative stress, mitochondrial dysfunction and apoptosis by acetyl-l-carnitine

Baghaei, A. and Solgi, R. and Jafari, A. and Abdolghaffari, A.H. and Golaghaei, A. and Asghari, M.H. and Baeeri, M. and Ostad, S.N. and Sharifzadeh, M. and Abdollahi, M. (2016) Molecular and biochemical evidence on the protection of cardiomyocytes from phosphine-induced oxidative stress, mitochondrial dysfunction and apoptosis by acetyl-l-carnitine. Environmental Toxicology and Pharmacology, 42. pp. 30-37.

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Abstract

The aim of the present study was to investigate the efficacy of acetyl-l-carnitine (ALCAR) on pathologic changes of mitochondrial respiratory chain activity, ATP production, oxidative stress, and cellular apoptosis/necrosis induced by aluminum phosphide (AlP) poisoning. The study groups included: the Sham that received almond oil only; the AlP that received oral LD50 dose of aluminum; the AC-100, AC-200, and AC-300 which received concurrent oral LD50 dose of AlP and single 100, 200, and 300mg/kg of ALCAR by intraperitoneal injection. After 24h, the rats were sacrificed; the heart and blood sample were taken for measurement of biochemical and mitochondrial factors. The results specified that ALCAR significantly attenuated the oxidative stress (elevated ROS and plasma iron levels) caused by AlP poisoning. ALCAR also increased the activity of cytochrome oxidase, which in turn amplified ATP production. Furthermore, flow cytometric assays and caspase activity indicated that ALCAR prohibited AlP-induced apoptosis in cardiomyocytes. © 2015 Elsevier B.V..

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: adenosine triphosphate; caspase 3; caspase 9; cytochrome c oxidase; levacecarnine; reactive oxygen metabolite; reduced nicotinamide adenine dinucleotide dehydrogenase (ubiquinone); succinate dehydrogenase (ubiquinone), animal cell; animal experiment; animal model; animal tissue; apoptosis; Article; blood sampling; cell protection; cell viability; controlled study; dose response; drug dose comparison; drug mechanism; enzyme activity; flow cytometry; heart muscle cell; intoxication; male; nonhuman; oxidative stress; priority journal; rat
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 07 Sep 2016 10:37
Last Modified: 13 Sep 2016 08:46
URI: http://eprints.goums.ac.ir/id/eprint/4578

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