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Simultaneous Determination of Nitrite and Nitrate in Milk Samples by Ion Chromatography Method and Estimation of Dietary Intake

Chamandust, S. and Mehrasebi, M.R. and Kamali, K. and Solgi, R. and Taran, J. and Nazari, F. and Hosseini, M.-J. (2016) Simultaneous Determination of Nitrite and Nitrate in Milk Samples by Ion Chromatography Method and Estimation of Dietary Intake. International Journal of Food Properties, 19 (9). pp. 1983-1993.

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Abstract

The presence of nitrate and nitrite in foods may be considered hazardous after ingestion in the gastrointestinal tract due to their reaction with naturally occurred secondary amines to form potentially carcinogenic nitrosamines. Due to this fact, a new method was developed in this study for the simultaneous determination of nitrite and nitrate in milk samples using by ion chromatography. Proposed mobile phase composed of sodium hydrogen carbonate and sodium carbonate (1.0 and 3.2 mmol/L) with a flow rate of 0.7 ml/min. The average recoveries for nitrate and nitrite were higher than 86 and 88, respectively. The limit of detection for nitrate and nitrite were 0.24 and 0.09 mg/L, respectively. The results of 102 real milk samples showed nitrate was found in all of the samples (100) with a mean of 34 ± 11 mg/L, while nitrite was found in none of the samples. The mean intake of nitrate in all age groups was lower than World Health Organization guideline. The present assessment concludes that the maximum contaminant level was equal to 82.8 mg/L nitrate. This method was fast, sensitive and accurate and is capable of being an alternative method in food control laboratories for investigation of nitrite and nitrate content. This is the first study of the determination and survey of nitrite and nitrate and exposure assessment of the Iranian population to nitrite and nitrate level in milk, which was widely used in infants and adolescents as one of the basic food components. Copyright © Taylor & Francis Group, LLC.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: Chromatography; Food supply; Ion chromatography; Ions; Nitrogen compounds; Sodium bicarbonate, Carcinogenic nitrosamines; Exposure assessment; Gastrointestinal tract; Maximum contaminant levels; Milk; Nitrites; Simultaneous determinations; World Health Organization, Nitrates
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 07 Sep 2016 09:13
Last Modified: 26 Sep 2016 08:07
URI: http://eprints.goums.ac.ir/id/eprint/4520

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