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An improved microculture method for diagnosis of cutaneous leishmaniasis

Pagheh, A. and Fakhar, M. and Mesgarian, F. and Gholami, S. and Ahmadpour, E. (2013) An improved microculture method for diagnosis of cutaneous leishmaniasis. Journal of Parasitic Diseases. pp. 1-5. ISSN 09717196 (ISSN) (In Press)

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Abstract

This study evaluated the performance of three diagnostic methods for cutaneous leishmaniasis (CL). Patients who came to the Health Center Laboratory of Gonbad-e-Qabus in Golestan Province, Iran, were enrolled in the study. Skin scraping smear, improved microculture (IMC) and polymerase chain reaction (PCR) were performed. A total of 303 subjects were recruited, among whom 273 subjects fulfilled the criteria for CL. Sensitivity and specificity were 88.8 % (95 % CI = 84.2-92.2 %) and 100.0 % for smears, 98.4 % (95 % CI = 96.1-99.1 %) and 100.0 % for IMC, both of them 100.0 % for PCR. Although, PCR was relatively more sensitive than the IMC, the high correlation (agreement = 96 %, Kappa = 0.82) between IMC and PCR along with the advantages of simplicity, rapidity, adequate sensitivity and as a needle free method, offers the IMC as a valuable alternative diagnostic method for PCR in diagnosis of CL. © 2013 Indian Society for Parasitology.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - J. Parasitic Dis. [Field not mapped to EPrints] AD - Toxoplasmosis Research Center, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran [Field not mapped to EPrints] AD - Department of Parasitology and Mycology, Molecular and Cell Biology Research Center, School of Medic, Mazandaran University of Medical Sciences, P.O. Box 48175-1665, Sari, Iran [Field not mapped to EPrints] AD - Gonbad Health Centre, Golestan University of Medical Sciences, Gonbad, Iran [Field not mapped to EPrints] AD - Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Cutaneous leishmaniasis, Diagnosis, Microculture, PCR, Skin scraping smear
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 19 Apr 2015 04:57
Last Modified: 12 Mar 2016 06:19
URI: http://eprints.goums.ac.ir/id/eprint/434

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