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Application of extreme environmental conditions to resuscitation of viable but non culturable E. coli DH5α

Heiat, M. and Mohkam, M. and Rad, S.A. and Jahromi, A.Y. and Eftekharei, M. and Ghasemi, Y. and Sadraeian, M. (2012) Application of extreme environmental conditions to resuscitation of viable but non culturable E. coli DH5α. European Journal of Experimental Biology, 2 (6). :2033-2037. ISSN 2248 –9215

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ABSTRACT The resuscitation of Viable But Non-Culturable (VBNC) state in Escherichia coli DH5α as one of the most usable expression host was investigated. The VBNC state in bacteria is defined as while the cells are alive but unable to grow visibly on nonselective growth medium. After collecting several samples, Non-culturable E. coli DH5α (that has undertaken on various recombinant manipulations) were divided into different groups in order to carry-out different experiments. They were treated to heat shock at 42ºC in different periods of time, different concentration of Bile-salts and NaCl and combinational of these methods. The results showed that the almost of resuscitation treatment had positive effect on reactivation of VBNC E. coli DH5α. The combination of these parameters (various NaCl and bile salts concentration and heat shock method at 42 °C in different time) in a binary manner, also inferred to suitable results. Furthermore, by applying the three stresses simultaneously we achieved optical density up to 0.58 and 9×108 CFU/ml which had presented the best results. The results show that by applying some alterations in the condition of such recombinant E. coli DH5α, the growth path of these bacteria which remain to a VBNC phase can be changed to the normal status. Key words: Non-culturable bacteria, E. coli, Bile-salts, NaCl, Thermal Shock.

Item Type: Article
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 05 May 2015 08:47
Last Modified: 20 Jun 2015 05:08
URI: http://eprints.goums.ac.ir/id/eprint/4159

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