Golestan University of Medical Sciences Repository

Genetic predisposition of interaction of HLA-DRB with heat shock protein 70-1 gene polymorphism in Iranian multiple sclerosis patients

Shahbazi, M. and Fathi, D. and Rashidbaghan, A. and Roshandel, D. (2008) Genetic predisposition of interaction of HLA-DRB with heat shock protein 70-1 gene polymorphism in Iranian multiple sclerosis patients. MULTIPLE SCLEROSIS, 14. S121.

Full text not available from this repository.


Background: Multiple sclerosis (MS) is a polygenic inflammatory disorder of the central nervous system most common in young women. Heat shock protein 70 (HSP70) is essential for maintenance of normal cellular function and recovery after insult. To date, there are some reports regarding the association between HSP70 gene polymorphisms and autoimmune disease, including MS. More recently, several researchers have focused on HLA-DRB alleles as a potential candidate. Most of them have shown that HLA-DRB1*15 allele is a higher genetic predisposition risk for development of MS. Objective: The aim of this study was to determine whether variation in the HSP70-1 gene at position +190 itself and amalgamation with HLA-DRB alleles is associated in MS patients and healthy individual controls. Methods: Genomic DNA was extracted from whole blood of 128 patients with MS and 137 age, ethnically and geographically matched control subjects. Patients were divided into two groups according to their expanded disability status scale score with mild to moderate and severe disease. The genotypes were determined by single specific primer-polymerase chain reaction. STATA software was used for analysis and Fisher’s exact test was applied to determine differences between groups. Results: Frequencies of HSP70-1 genotypes were significantly different between MS patients and control subjects (P =0.004; OR= 2.87; CI=1.55–5.33). In addition, G/G genotype was protective when come with HLA-DRB1*13, *14, *16, DRB3*01 and DRB5*01 alleles. Conversely, G/G genotype along with DRB1*0103 allele was more frequent in MS patients. Furthermore, age at onset and course of the disease were influenced by interaction of HLA-DRB alleles and HSP70-1 gene polymorphism. Conclusions: Our results have shown that HSP70-1 gene (G/C) polymorphism was strongly associated with development of MS in this population. Interestingly, the combination of G/G genotype with DRB1*13, *14, *16, DRB3*01 and DRB5*01 alleles enhanced protection against MS; in contrast, combination of DRB1*0103 allele with G/G genotype increased susceptibility to MS.

Item Type: Article
Additional Information: 13th Annual Meeting of the Amer-Comm-for-Treatment-and-Res-in-Multiple-Sclerosis/24th Congress of the European-Comm-for-Treatment-and-Res-in-Multiple-Sclerosis/5th Congress of the Latin-Amer-Comm-for-Treatment-and-Res-in-Multiple-Sclerosis, Montreal, CANADA, SEP 17-20, 2008
Subjects: موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 27 Apr 2015 10:42
Last Modified: 25 Jun 2017 04:31
URI: http://eprints.goums.ac.ir/id/eprint/4088

Actions (login required)

View Item View Item