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The apoptotic and inhibitory effects of Polygonum avicular extract on Hela-S cervical cancer cell line

Fazli, D. and Rahmati, M. and Bannazadeh, H. and Rasmi, Y. and Farrokhi, A. and Ahmadian, N. and Khalaj, M. (2011) The apoptotic and inhibitory effects of Polygonum avicular extract on Hela-S cervical cancer cell line. CLINICAL BIOCHEMISTRY, 44 (13, S). S114.

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Abstract

Introduction: Cervical cancer is the second leading cause of cancer death in women. There are different methods for cancer cell inhibition. Some medicinal plants have anti-cancer activity, but lack of scientific evidence in connection with mechanism of action of medicinal plants that reduced their clinical applications. In the present study apoptotic and inhibitory effects of Polygonum avicular extract effects on cervical cancer cell line were investigated. Methods: After extraction of P. avicular in three phase (n-hezane, dichloromethane and methanol), its cytotoxicity was evaluated by MTT assay and IC50 determined at different times (12, 24 and 48 h). Then apoptotic effect determined by annexin v method. Results: The effect of P. avicular extracts on Hela-S cervical cancer cell apoptosis and cell cytotoxicity was demonstrated. IC50 of methanolic extract of P. avicular was 0.28 mg/ml, 0.27 mg/ml and 0.41 mg/ml for 12 h, 24 h and 48 h by MTT assays, respectively. Cytotoxicity of methanolic extract of P. avicular was dose-dependent and time dependent. Also, methanolic extract of P. avicular cause apoptosis time-dependently. Discussion: In conclusion, current work demonstrated that methanolic extract of P. avicular has potent anti-growth effect on Hela-S and time and dose-dependently induces apoptosis in this cell line as in vitro model of cervical cancer. Therefore, P. avicular can be natural potent chemopreventive and chemotherapeutic plant for cervical cancer patients and constituents of its methanolic extract can be appropriate candidate for drug development.

Item Type: Article
Uncontrolled Keywords: Polygonum avicular; Hela-S cell line; Apoptosis
Subjects: موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 27 Apr 2015 09:40
Last Modified: 20 Jun 2017 03:57
URI: http://eprints.goums.ac.ir/id/eprint/3943

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