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Differentiation of Entamoeba spp. in children With dysentery in Gorgan, Iran

Sharbatkhori, M. and Cheraghali, F. and Nazemalhosseini Mojarad, E. and Maghsoodloorad, F.S. and Taherkhani, H. and Vakili, M. (2014) Differentiation of Entamoeba spp. in children With dysentery in Gorgan, Iran. CLINICAL BIOCHEMISTRY, 47 (9). p. 780.

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Abstract

Background: Differential detection of two morphologically indistinguishable protozoan parasites Entamoeba histolytica and Entamoeba dispar has a great clinical and epidemiological importance because of potential invasive pathogenic E. histolytica and non-invasive parasite E. dispar. The present study was performed in order to differentiate E. histolytica and E. dispar in children from Gorgan City, using a PCR method. Methods: One hundred and five dysentery samples were collected from children hospitalized in Taleghani Hospital in Gorgan City. The fecal specimens were examined by light microscopy (40×) to distinguish Entamoeba complex. A single round PCR amplifying partial small-subunit rRNA gene was performed on positive microscopy samples to differentiate E. histolytica, E. dispar and Entamoeba moshkovskii from each other. Results: Twenty five specimens (23.8%) were positive for Entamoeba complex in direct microscopic examination. PCR using positive controls indicated E. histolytica and E. dispar in two (2/25, 8%) and three (3/25, 12%) samples, respectively. Conclusion: There is a warrant to perform molecular diagnosis for stool examination at least in hospitalized children in order to prevent incorrect reports from laboratories and consequently mistreatment by physicians.

Item Type: Article
Subjects: موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 27 Apr 2015 08:04
Last Modified: 25 Jun 2017 05:54
URI: http://eprints.goums.ac.ir/id/eprint/3709

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