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Effect of gestational diabetes on purkinje and granule cells distribution of the rat cerebellum in 21 and 28 days of postnatal life

Razi, E.M. and Ghafari, S. and Golalipour, M.J. (2015) Effect of gestational diabetes on purkinje and granule cells distribution of the rat cerebellum in 21 and 28 days of postnatal life. Basic and Clinical Neuroscience, 6 (1). pp. 6-13.

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Abstract

Introduction: Diabetes mellitus is associated with nervous system alterations in both human and animal models. This study was done to determine the effect of gestational diabetes on the Purkinje and granular cells in the cerebellum of rat offspring. Methods: 10 Wistar rats Dams were randomly allocated in control and diabetic group. The experimental group received 40 mg/kg/body weight of streptozotocin (STZ) at the first day of gestation and control groups received saline injection intraperitoneally (IP). Six male offsprings of gestational diabetic mothers and control dams, at the 21, 28 postnatal days were randomly scarified and coronal sections of cerebellum (6 micrometer) serially collected. The neurons were stained with cresyl violet. Results: The Purkinje cells density in the apex and depth of cerebellum in P21, in the experimental group was reduced 23 and 15 in comparison with the control group (P<0.001). The granular cells density in the experimental group was reduced 19.58 and 18.3 in comparison with the controls (P<0.001). The Purkinje cells density of cerebellum in P28, in the diabetic group reduced to 22.12 and 12.62 in comparison with the control group (P<0.001). The granular cells density in the diabetic group reduced 17.14 and 16.12 in comparison with the control group (P<0.001). Discussion: The Purkinje and granular cells significantly reduced in gestational diabetes rat offspring.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: glucose, animal cell; animal experiment; animal model; animal tissue; Article; cell count; cell density; cellular distribution; cerebellum; cerebellum cortex; controlled study; cortical thickness (brain); female; glucose blood level; granule cell; histopathology; morphometrics; nonhuman; pregnancy diabetes mellitus; progeny; Purkinje cell; rat
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 20 Apr 2015 08:51
Last Modified: 22 Jul 2017 08:53
URI: http://eprints.goums.ac.ir/id/eprint/2493

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