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Comparison of four methods for isolation of Yersinia enterocolitica from raw and pasteurized milk from northern Iran

Soltan-Dallal, M.-M. and Tabarraie, A. and Moezardalan, K. (2004) Comparison of four methods for isolation of Yersinia enterocolitica from raw and pasteurized milk from northern Iran. International Journal of Food Microbiology, 94 (1). pp. 87-91. ISSN 01681605 (ISSN)

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Abstract

Four methods for isolation of Yersinia enterocolitica from raw and pasteurized milk from northern Iran were compared. Three hundred and ten raw milk samples were collected from tanks on their arrival at various central dairies, and 40 pasteurized milk samples were collected from tanks on their arrival at a manufacturing plant. Each sample was examined for the presence of Y. enterocolitica by (1) direct culture; (2) enrichment in double-strength buffered peptone water at 4°C for 1 month; (3) enrichment in modified Rappaport medium at room temperature for 72 h after a preenrichment in double-strength peptone water at 4°C for 1 month; and (4) enrichment in a medium containing sucrose, tris (hydroxymethyl) aminomethane, sodium azide, and ampicillin at 28°C for 48 h after a preenrichment in double-strength peptone water at 4°C for 1 month. All samples and enrichments were spread on MacConkey agar plus calcium chloride and Tween 80, Yersinia selective agar, and Hektoen medium plus ampicillin. Five samples (1.6%) of raw milk but no pasteurized milk samples were positive for Y. enterocolitica. No Y. enterocolitica were recovered by methods 1 or 2. Y. enterocolitica were recovered from 2 samples by method 3 followed by culture on Yersinia selective agar, and from 5 samples by method 4 followed by culture on Hektoen medium plus ampicillin. The isolates were biotype 1A or 1B, serotype O:7-13 or O:9 and phage type Xo or Xz. All isolates were resistant to ampicillin and amoxicillin, and sensitive to tetracycline, streptomycin, chloramphenicol, and trimethoprim-sulfamethoxazole. © 2004 Elsevier B.V. All rights reserved.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Int. J. Food Microbiol. [Field not mapped to EPrints] C2 - 15172488 [Field not mapped to EPrints] AD - Department of Microbiology, School of Public Health, Tehran Univ. of Medical Sciences, P.O. Box 6446, Tehran 14155, Iran [Field not mapped to EPrints] AD - Gorgan Univ. of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Natl. Res. Dept. of Foodborne Dis., Shaheed Beheshti Univ. of Med. Sci., Taleghani Hospital, Tabnak St., Evin, Tehran 19857, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Iran, Milk, Recovery, Yersinia enterocolitica, agar, amoxicillin, ampicillin, calcium chloride, chloramphenicol, cotrimoxazole, peptone, polysorbate 80, sodium azide, streptomycin, sucrose, tetracycline, trometamol, water, article, bacteriophage typing, bacterium culture, bacterium isolate, bacterium isolation, controlled study, culture medium, intermethod comparison, milk, nonhuman, room temperature, serotype, Yersinia enterocolitica, Animals, Anti-Bacterial Agents, Colony Count, Microbial, Consumer Product Safety, Culture Media, Drug Resistance, Bacterial, Food Contamination, Food Handling, Humans, Iran, Microbial Sensitivity Tests, Milk, Serotyping, Temperature, Time Factors, Yersinia enterocolitica, Yersinia, Yersinia enterocolitica
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 18 Apr 2015 05:14
Last Modified: 04 Feb 2017 11:17
URI: http://eprints.goums.ac.ir/id/eprint/2470

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