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Combining mammaglobin and carcinoembryonic mRNA markers for early detection of micrometastases from breast cancers - a molecular study of 59 patients

Ghaffari, S.R. and Sabokbar, T. and Tahmasebi, S. and Dastan, J. and Shorakae, S. and Moradi, A. and Tirgari, F. and Mohagheghi, M.A. and Mosavi-Jarrahi, A. (2006) Combining mammaglobin and carcinoembryonic mRNA markers for early detection of micrometastases from breast cancers - a molecular study of 59 patients. Asian Pacific Journal of Cancer Prevention, 7 (3). pp. 396-398. ISSN 15137368 (ISSN)

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Abstract

Introduction: As many as 30% of node-negative breast cancer patients relapse within five years, suggesting that current histological detection methods are inadequate for identifying metastatic disease. Detecting small number of cancer cells in the breast tissue or lymph node by reverse transcription-polymerase chain reaction (RT-PCR) assays using a combination of tissue and cancer specific markers might be very useful in the early detection or monitoring of the treatment. Mammaglobin is a member of the uteroglobin gene family and appears to be expressed only in breast tissue. Carcinoembryonic antigen has been the preferred molecular marker for detection of micro metastases in lymph nodes in almost all carcinomas. Materials and Methods: Samples were collected from randomly chosen breast cancer patients undergoing modified mastectomy or breast conserving surgery between September 2003 and July 2004. RT-PCR was applied to study the expression of MMG and CEA markers. Breast cancer micrometastases in axillary lymph nodes were also assessed. Results: The MMG marker was positive in 9/10 normal breast tissues, 3/ 3 breast fibroadenomas and 37/39 of breast carcinoma tissues, giving an overall sensitivity of 94%. The sensitivity was 80% for metastatic lymph node samples. On the other hand CEA showed 95% sensitivity for malignant breast tumors and 100% sensitivity for metastatic lymph nodes. Conclusions: RT-PCR using a combination of MMG and CEA markers is a powerful tool to complement current routine histopathology techniques for detection of breast cancer metastasis in axillary nodes.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Asian Pac. J. Cancer Preven. [Field not mapped to EPrints] C2 - 17059329 [Field not mapped to EPrints] AD - Cancer Institute Research Center, Tehran University of Medical Sciences, Iran [Field not mapped to EPrints] AD - Department of Cancer Research, Gene Institute, Iran [Field not mapped to EPrints] AD - Department of Medical Genetics, Tehran University of Medical Sciences, Iran [Field not mapped to EPrints] AD - Department of Molecular Virology, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Department of Pathology, Cancer Institute of Iran, Tehran University of Medical Sciences, Iran [Field not mapped to EPrints] AD - Department of Surgical Oncology, Cancer Institute of Iran, Tehran University of Medical Sciences, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Breast cancer, Carcinoembryonic Antigen (CEA) Genes, Mammaglobin (MMG), RT-PCR, carcinoembryonic antigen, mammaglobin, messenger RNA, RNA, tumor marker, tumor protein, uteroglobin, article, axilla, breast tumor, clinical trial, controlled clinical trial, controlled study, early diagnosis, female, fibroadenoma, genetics, human, lymph node, lymph node metastasis, mastectomy, metabolism, pathology, randomized controlled trial, reverse transcription polymerase chain reaction, sensitivity and specificity, Axilla, Breast Neoplasms, Carcinoembryonic Antigen, Early Diagnosis, Female, Fibroadenoma, Humans, Lymph Nodes, Lymphatic Metastasis, Mastectomy, Neoplasm Proteins, Reverse Transcriptase Polymerase Chain Reaction, RNA, Messenger, RNA, Neoplasm, Sensitivity and Specificity, Tumor Markers, Biological, Uteroglobin
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 15 Apr 2015 09:16
Last Modified: 06 Feb 2017 08:03
URI: http://eprints.goums.ac.ir/id/eprint/2423

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