Golestan University of Medical Sciences Repository

Alterations in plasma lipid peroxidation and total antioxidant status during storage of blood

Marjani, A. (2006) Alterations in plasma lipid peroxidation and total antioxidant status during storage of blood. Pakistan Journal of Biological Sciences, 9 (13). pp. 2520-2523. ISSN 10288880 (ISSN)

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Abstract

The aim of the present study was designed to determine the alteration of plasma lipid peroxidation-(by measuring the level of MDA) and total antioxidant status in stored blood. This study is carried out to find out the quantitative alterations and the useful length of stored blood. The whole blood were taken from 10 donors. Red Blood Cells (RBC) were counted in whole blood. The levels of Potassium (P) and lactate dehydrogenate activity(LDH) were measured in plasma for determination of hemolysis. The levels of malondialdehyde (MDA) and Total Antioxidant Status (TAS) were studied in plasma for determination of lipid peroxidation and antioxidant system, respectively. The measurement were performed at the day 0, 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33 and 35 of the storage. The levels of malondialdehyde and Potassium and lactate dehydrogenate activity increased (p<0.05) depending on storage time whereas Total Antioxidant Status (TAS) and Red Blood Cells decreased (p<0.05). The alterations of MDA, TAS, P, LDH and RBC in the measurement days were as follows: MDA, P and LDH significantly increased at the day 9, 5 and 5 whereas TAS and RBC decreased at the day 13 and 29, respectively. These results suggest that increased level of MDA and decreased level of TAS in the stored blood can not improve the vialibility and longevity of RBC by increasing cell damage caused by free radicals at the day mentioned above. To improve the quality of stored blood is to supplement blood donors with antioxidants and vitamins at least one week before blood collection to keep and store the stored blood for longer time until using for transfusion. We conclude that increased level of MDA and decreased TAS in stored blood which can cause the beginning of hemolysis. It is therefore necessary to control these factors before blood transfusion. © 2006 Asian Network for Scientific Information.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Pak. J. Biol. Sci. [Field not mapped to EPrints] AD - Department of Biochemistry and Biophysics, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Lipid peroxidation-total antioxidant status-blood storage
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 19 Apr 2015 07:41
Last Modified: 06 Feb 2017 05:45
URI: http://eprints.goums.ac.ir/id/eprint/2420

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