Golestan University of Medical Sciences Repository

Effects modification of iron hematoxylin on neuron staining

Gharravi, A.M. and Golalipour, M.J. and Ghorbani, R. and Khazaei, M. (2007) Effects modification of iron hematoxylin on neuron staining. Pakistan Journal of Biological Sciences, 10 (5). pp. 768-772. ISSN 10288880 (ISSN)

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Abstract

Iron weigert staining methods is used as nuclear staining. In present study we introduce a modification iron weigert hematoxylin for staining neuron without astrocytes. Whole brain of adult wistar rats (12-13 week old) were removed, immersed in formaldehyde fixative and embedded in paraffin. Sections, 5-7 μm (from brain cortex, hippocampus, cerebellum) divided to three groups: one for staining by Hematoxyllin and eosin, second for staining by cresyl fast violet (that specially performed for Nis1 substances in neuron) and last for staining by modification iron hematoxyllin methods, but different in quantity and quality. In new method general and specific architecture of neuron, nucleus and nuclear envelope was clearly visible reactions of neuron were predominant. Astrocyte did not respond to staining methods. Also spines (axon) of purkinje cells clearly visible. Modification iron weigert hematoxylin can be replacement to cost and time consuming chemical staining method for staining neurons. © 2007 Asian Network for Scientific Information.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Pak. J. Biol. Sci. [Field not mapped to EPrints] C2 - 19069861 [Field not mapped to EPrints] AD - Department of Anatomy, Gorgan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Reproductive Research Center, Kerman Shah University of Medical Sciences, Kerman Shah, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Astrocyte, Iron hematoxylin, Neuron, hematoxylin, animal, article, brain, chemistry, cytology, male, metabolism, nerve cell, rat, staining, Wistar rat, Animals, Brain, Hematoxylin, Male, Neurons, Rats, Rats, Wistar, Staining and Labeling, Rattus norvegicus
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 20 Apr 2015 03:37
Last Modified: 11 Feb 2017 08:44
URI: http://eprints.goums.ac.ir/id/eprint/2406

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