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Formaldehyde exposure induces histopathological and morphometric changes in the rat testis

Golalipour, M.J. and Azarhoush, R. and Ghafari, S. and Gharravi, A.M. and Fazeli, S.A. and Davarian, A. (2007) Formaldehyde exposure induces histopathological and morphometric changes in the rat testis. Folia Morphologica, 66 (3). pp. 167-171. ISSN 00155659 (ISSN)

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Abstract

Formaldehyde is a chemical which is traditionally used for fixing cadavers and routine histopathology techniques. It is vaponsed during the dissection and practical study of a cadaver. Previous studies have shown that this vapour may cause clinical symptoms such as throat, eye, skin and nasal irritation. This study was designed to determine the histopathology and morphometrics of the rat testis when all the experimental animals were exposed to formaldehyde for 18 weeks. The study was performed in 2004 on 28 albino Wistar rats of 6-7 postnatal weeks. The rats were divided into three case groups (E1: 4 h/d, 4 d/w; E2: 2 h/d, 4 d/w; E3: 2 h/d, 2 d/w) and one control group. The testes specimens were sectioned at 5 μm and stained with the haematoxylin and eosin staining technique for histological and morphometrical studies. We found a severe decrease in germ cells associated with spermatogenesis arrest in the E1 group. A decrease in germ cells and a thickening of the basal membrane of the seminiferous tubules were seen in E2. Displacement of Sertoli and germinal cells were also found in the E3 group. The mean seminiferous tubular diameter and seminiferous epithelial height in the experimental groups were decreased in comparison with the control group and the differences were statistically significant (p < 0. 05). The findings of this study revealed that chronic formaldehyde exposure can cause histopathological and morphometric changes to the seminiferous epithelium in rats and that these changes depend on the duration of the formaldehyde exposure. Copyright © 2007 Via Medica.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Folia Morphol. [Field not mapped to EPrints] C2 - 17985313 [Field not mapped to EPrints] AD - Department of Histology and Embryology, Gorgan University of Medical Sciences, P.O. Box 49175-553, Gorgan, Iran [Field not mapped to EPrints] AD - Department of Pathology, Gorgan University of Medical Sciences, P.O. Box 49175-553, Gorgan, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Duration, Germ cell, Seminiferous epithelial height, Seminiferous epithelium diameter, Sertoli, Spermatogenesis, eosin, formaldehyde, hematoxylin, animal experiment, animal model, animal tissue, article, basement membrane, cadaver, controlled study, dissection, experimental animal, germ cell, histopathology, long term exposure, male, morphometrics, nonhuman, rat, seminiferous tubule, Sertoli cell, spermatogenesis, staining, statistical significance, testis, tissue fixation, tissue section, vapor, vaporization, Wistar rat, Animals, Dose-Response Relationship, Drug, Fixatives, Formaldehyde, Histological Techniques, Male, Rats, Rats, Wistar, Seminiferous Epithelium, Sertoli Cells, Spermatozoa, Testis, Animalia, Rattus, Rattus norvegicus
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 18 Apr 2015 04:20
Last Modified: 13 Feb 2017 07:19
URI: http://eprints.goums.ac.ir/id/eprint/2379

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