Golestan University of Medical Sciences Repository

Detection of human papillomavirus DNA sequences in oral lesions using polymerase chain reaction

Zarei, M.R. and Moradie, A. and Hamkar, R. and Mohammadalizadeh, S. and Chamani, G. and Alizadeh, N. and Kakooei, S. and Eslami, B. (2007) Detection of human papillomavirus DNA sequences in oral lesions using polymerase chain reaction. Acta Medica Iranica, 45 (3). pp. 177-182. ISSN 00446025 (ISSN)

[img]
Preview
PDF - Published Version
Download (374kB) | Preview

Abstract

The purpose of the present study was to estimate the frequency of HPV DNA in four groups of oral lesions, including oral squamous cell carcinoma. Sixty paraffin-embedded oral tissue samples were examined for the presence of HPV DNAs using the PCR technique. These specimens were obtained from patients with oral squamous cell carcinoma (OSCC), leukoplakia, oral lichen planus (OLP), and pyogenic granuloma (PG). Consensus primers for L1 region (MY09 and MY11) and specific primers were used for detection of HPV DNA sequences in this study. we detected HPV DNA in 60% (9 out of 15) of OSCCs, 26.7% (4 out of 15) of leukoplakia, 13.3% (2 out of 15) of OLPs, and 6.7% (1 out of 15) of PGs. Statistical analysis showed that the prevalence of HPV in OSCC was significantly higher than other groups (P < 0.05). The frequency of HPV-16 and 18 detection in OSCC samples were 40% and 20%, respectively. The prevalence of these high risk HPVs was significantly higher in OSCC group (P < 0.05). The results of the present study show a successive increase of detection rate of HPV-16 and 18 DNAs from low level in samples of pyogenic granuloma and non-premalignant or questionably premalignant lesions of OLP to premalignant leukoplakia and to OSCC. © 2007 Tehran University of Medical Sciences. All rights reserved.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Acta Med. Iran. [Field not mapped to EPrints] AD - Department of Oral Medicine, School of Dentistry, Kerman University of Medical Sciences, Kerman, Iran [Field not mapped to EPrints] AD - Department of Virology, School of Medicine, Gorgan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Department of Virology, School of Medicine, Medical Sciences/University of Tehran, Tehran, Iran [Field not mapped to EPrints] AD - Department of Nursing, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran [Field not mapped to EPrints] AD - Department of Oral Medicine, School of Dentistry, Ghazvin University of Medical Sciences, Ghazvin, Iran [Field not mapped to EPrints] AD - Department of Oral Pathology, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran [Field not mapped to EPrints] AD - School of Dentistry, Shafa Ave, Kerman 7618759689, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Human papillomavirus, Leukoplakia, Lichen planus, Oral squamous cell carcinoma, Polymerase chain reaction, paraffin, virus DNA, adult, aged, article, consensus, controlled study, DNA sequence, female, frequency analysis, human, human tissue, leukoplakia, lichen planus, major clinical study, male, mouth carcinoma, mouth lesion, Papilloma virus, polymerase chain reaction, prevalence, pyogenic granuloma, squamous cell carcinoma, statistical analysis, tissue section, virus detection
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 15 Apr 2015 04:24
Last Modified: 11 Feb 2017 08:11
URI: http://eprints.goums.ac.ir/id/eprint/2361

Actions (login required)

View Item View Item