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Quantitative studies of hepatocytes in periportal and perivenous zones of the liver lobules in diabetic rats

Golalipour, M.J. and Ghafari, S. (2007) Quantitative studies of hepatocytes in periportal and perivenous zones of the liver lobules in diabetic rats. International Journal of Zoological Research, 3 (4). pp. 186-192. ISSN 18119778 (ISSN)

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Abstract

This study was designed to evaluate the morphometric changes of parenchymal cells in the periportal (Z1) and perivenous (Z3) zones of the liver lobules that occur after Streptozotocin-induced diabetes in rats. Twenty male rats were allocated in two groups of normal and diabetic. Hyperglycemia in rats induced by 80 mg kg-1 Streptozotocin intraperitoneally. The blood glucose concentration was measured by using a Glucometer in 1st, 3rd and 5th week. After five weeks, with using anesthesia liver removed. Liver specimens were fixed in buffered formaldehyde and embedded in paraffin. Hematoxylin and eosin stained sections were used for quantitative morphometric analysis. Morphometric parameters in mononuclear cells were measured by Olysia software. All data are shown as mean with standard error of means and analyzed using the Student's t-test and p-value less of 0.05. Mean areas of hepatocyte, their nuclei and nucleolus were reduced by approximately 1.29, 7.27 and 0.76% in zone 1 in the diabetic rats in comparison with the control group. While mean areas of hepatocyte, their nuclei and nucleolus were increased by approximately 5.26, 2.53 and 3.10%, respectively, in zone 3 in the diabetic and control rats. It is concluded that Streptozotocin injection leads to reduction in area of hepatocytes and their nuclei and nucleolar in zone 1 and an increase in area of hepatocytes and their nuclei and nucleolar in zone 3. © 2007 Academic Journals Inc.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Int. J. Zool. Res. [Field not mapped to EPrints] AD - Department of Histology and Embryology, Gorgan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Department of Histology and Embryology, Gorgan University of Medical Sciences, P.O. Box 49175-553, Gorgan, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Diabetes, Hepatocyte, Liver, Morphometry, Rat, Streptozotocin, Rattus
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 18 Apr 2015 05:28
Last Modified: 15 Feb 2017 07:44
URI: http://eprints.goums.ac.ir/id/eprint/2353

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