Golestan University of Medical Sciences Repository

Nanodiagnostic method for colorimetric detection of Mycobacterium tuberculosis 16S rRNA

Gill, P. and Ghalami, M. and Ghaemi, A. and Mosavari, N. and Abdul-Tehrani, H. and Sadeghizadeh, M. (2008) Nanodiagnostic method for colorimetric detection of Mycobacterium tuberculosis 16S rRNA. Nanobiotechnology, 4 (1-4). pp. 28-35. ISSN 15511286 (ISSN)

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Abstract

A nanodiagnostic method using nucleic acid sequence-based amplification (NASBA) and gold nanoparticle probes (AuNP probes) was developed for colorimetric detection of Mycobacterium tuberculosis. The primers targeting 16S rRNA were used for the amplification of mycobacterial RNA by the isothermal NASBA process. The amplicons were hybridized with specific gold nanoparticle probes. The RNA-DNA hybrids were colorimetrically detected by the accumulation of gold nanoparticles. Using this method, 10 CFU ml-1 of M. tuberculosis was detected within less than 1 h. Results obtained from the clinical specimens showed 94.7% and 96% sensitivity and specificity, respectively. No interference was encountered in the amplification and detection of M. tuberculosis in the presence of non-target bacteria, confirming the specificity of the method. © 2009 Humana Press Inc.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Nanobiotechnology [Field not mapped to EPrints] AD - Department of Nanobiotechnology, Faculty of Basic Sciences, Tarbiat Modares University, 14115-175, Tehran, Iran [Field not mapped to EPrints] AD - Laboratory Part of Center of Disease Control (CDC), Deputy of Health, Ministry of Health Care and Medical Education, Tehran, Iran [Field not mapped to EPrints] AD - Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran [Field not mapped to EPrints] AD - PPD Department, Razi Institute, Karaj, Iran [Field not mapped to EPrints] AD - Department of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran [Field not mapped to EPrints] AD - Faculty of Medicine, Golestan University of Medical Sciences and Health Care, Gorgan, Iran [Field not mapped to EPrints] AD - Department of Genetics, Faculty of Basic Sciences, Tarbiat Modares University, Tehran, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: 16S rRNA, Gold nanoparticle probes, Mycobacterium tuberculosis, Nanodiagnostic method, NASBA, gold nanoparticle, RNA 16S, amplicon, article, bacterium identification, colony forming unit, colorimetry, controlled study, DNA RNA hybridization, Escherichia coli, gene amplification, gene targeting, molecular probe, Mycobacterium tuberculosis, nonhuman, nucleotide sequence, priority journal, RNA analysis, sensitivity and specificity, Mycobacterium tuberculosis
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 19 Apr 2015 05:41
Last Modified: 26 Feb 2017 05:37
URI: http://eprints.goums.ac.ir/id/eprint/2306

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