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The gaseous mediator, hydrogen sulphide, inhibits in vitro motor patterns in the human, rat and mouse colon and jejunum

Gallego, D. and Clavé, P. and Donovan, J. and Rahmati, R. and Grundy, D. and Jiménez, M. and Beyak, M.J. (2008) The gaseous mediator, hydrogen sulphide, inhibits in vitro motor patterns in the human, rat and mouse colon and jejunum. Neurogastroenterology and Motility, 20 (12). pp. 1306-1316. ISSN 13501925 (ISSN)

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Abstract

Hydrogen sulphide (H2S) has been recently proposed as a transmitter in the brain and peripheral tissues. Its role in the gastrointestinal tract is still unknown despite some data which suggest an involvement mediating smooth muscle relaxation. The aim of this study was to investigate the effect of this gas on intestinal segments from mouse jejunum and colon, and muscular strips from the human and rat colon. In isolated segments of mouse colon and jejunum, bath applied sodium hydrogen sulphide (NaHS) (a H2S donor) caused a concentration-dependent inhibition of spontaneous motor complexes (MCs) (IC50 121 μmol L-1 in the colon and 150 μmol L-1 in the jejunum). This inhibitory effect of NaHS on MCs was (i) unaffected by tetrodotoxin (TTX), capsaicin, pyridoxal-phosphate- 6-azophenyl-2',4'-disulfonate and N-nitro-l-arginine suggesting a non-neural effect and (ii) significantly reduced by apamin 3 μmol L -1. NaHS concentration-dependently inhibited the spontaneous motility in strips from human colon (IC50 261 μmol L-1) and rat colon (IC50 31 μmol L-1). The inhibitory effect of NaHS on colonic strips was (i) unaffected by the neural blocker TTX (1 μmol L-1) with IC50 183 μmol L-1 for the human colon and of 26 μmol L-1 for the rat colon and (ii) significantly reduced by glybenclamide (10 μmol L-1), apamin (3 μmol L -1) and TEA (10 mmol L-1) with IC50 values of 2464, 1307 and 2421 μmol L-1 for human strips, and 80, 167 and 674 μmol L-1 for rat strips respectively. We conclude that H 2S strongly inhibits in vitro intestinal and colonic motor patterns. This effect appears to be critically dependent on K channels particularly apamin-sensitive SK channels and glybenclamide-sensitive K (ATP) channels. © 2008 The Authors.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Neurogastroenterol. Motil. [Field not mapped to EPrints] C2 - 19019033 [Field not mapped to EPrints] AD - Department of Cell Biology, Physiology and Immunology, Edifici V, Universidad Autonoma de Barcelona, 08193 Bellaterra, Barcelona, Spain [Field not mapped to EPrints] AD - Gastrointestinal Diseases Research Unit (GIDRU), GIDRU Wing, Kingston General Hospital, 76 Stuart St, Kingston, ON K7M 2V7, Canada [Field not mapped to EPrints] AD - Department of Cell Biology, Physiology and Immunology, Universitat Autònoma de Barcelona (UAB), Bellaterra, Barcelona, Spain [Field not mapped to EPrints] AD - Centro de Investigacion Biomedica en Red de Enfermedades Hepaticas Y Digestivas (Ciberehd), Instituto de Salud Carlos III, Barcelona, Spain [Field not mapped to EPrints] AD - Department of Surgery, Hospital de Mataró, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain [Field not mapped to EPrints] AD - Fundació de Gastroenterologia Dr. F. Vilardell, Barcelona, Spain [Field not mapped to EPrints] AD - Department of Biomedical Science, University of Sheffield, Sheffield, United Kingdom [Field not mapped to EPrints] AD - Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Gastrointestinal Diseases Research Unit (GIDRU), Departments of Medicine and Physiology, Queen's University, Kingston, ON, Canada [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Cystathionine β-synthase, Cystathionine γ-lyase, Gastrointestinal tract, Hydrogen sulphide, Potassium channels, Smooth muscle, apamin, capsaicin, glibenclamide, hydrogen sulfide, n(g) nitroarginine methyl ester, nitric oxide, potassium channel, purinergic P2 receptor, pyridoxal phosphate 6 azophenyl 2',4' disulfonic acid, tetrodotoxin, tetrylammonium chloride, vanilloid receptor 1, adult, aged, animal experiment, animal tissue, article, colon motility, concentration (parameters), controlled study, female, human, human tissue, in vitro study, intestine contraction, jejunum, male, motor activity, mouse, nonhuman, prepulse inhibition, priority journal, protein function, rat, smooth muscle contraction, smooth muscle relaxation, Aged, Animals, Colon, Female, Gastrointestinal Motility, Humans, Hydrogen Sulfide, Jejunum, Male, Mice, Middle Aged, Myoelectric Complex, Migrating, Neurotransmitter Agents, Organ Culture Techniques, Potassium Channels, Rats, Rats, Sprague-Dawley
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 19 Apr 2015 05:50
Last Modified: 26 Feb 2017 09:40
URI: http://eprints.goums.ac.ir/id/eprint/2305

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