Golestan University of Medical Sciences Repository

Association between Endothelial Selectin (E-selectin) gene polymorphisms and E-selectin level with visceral leishmaniais, in an ARMS-PCR based study

Fallah, M. and Bazmani, A. and Mazloumi Gavgani, A. and Taherkhani, H. and Hajilooi, M. (2008) Association between Endothelial Selectin (E-selectin) gene polymorphisms and E-selectin level with visceral leishmaniais, in an ARMS-PCR based study. Iranian Journal of Parasitology, 3 (4). pp. 23-31. ISSN 17357020 (ISSN)

[img]
Preview
PDF - Published Version
Download (386kB) | Preview

Abstract

Background: In the visceral leishmaniasis (VL), parasites reside in reticuluendothelial system, mainly in macrophages. Endothelial Selectin (E-selectin) might play an important role in leukocyte-endothelium interactions and inflammatory cell recruitment. The aim of this study was determining E-selectin level and its polymorphism in three groups, patients, seropositive and healthy individuals. Methods: Serum soluble E-selectin levels as well as 2 polymorphisms of E-selectin (Ser128Arg and Leu554Phe) were measured in a cohort of patients with documented VL (n=64), a healthy control group (n=74) and a seropositive for VL but without any symptoms (n=81). Circulation concentration of E-selectin levels was measured by ELIS. The amplification refractory mutation system (ARMS)-PCR procedure was used for detecting polymorphisms. Results: The mean of E-selectin levels significantly differed between three groups (P<0.026), and were increased in patients in comparison with other groups. Difference was more considerable between two groups of patients and healthy ones (patients 92.8 ng/ml; healthy individuals 71.9 ng/ml). Polymorphisms were associated with soluble E-selectin levels and altogether explained 14.4%, 7.2%, and 8.7% in patients, seropositive and seronegative healthy individuals, respectively. Distribution of polymorphisms of 128Ser/Arg and 554Leu/Phe among three groups was not different significantly; however, there was a considerable arrangement in distribution of Ser128Arg polymorphism and 128Arg allele in healthy group was more than two fold of patients (55% against 20%). Conclusion: The association between soluble E-selectin levels and visceral leishmaniasis suggests that this molecule might have significant role in the inflammatory process in VL. Moreover, frequency of 128Arg allele in healthy group was higher than patients.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Iran. J. Parasitol. [Field not mapped to EPrints] AD - Dept. of Parasitology and Mycology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran [Field not mapped to EPrints] AD - Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran [Field not mapped to EPrints] AD - Dept. of Parasitology, Research Center of Infectious Diseases, Tabriz University of Medical Sciences, Tabriz, Iran [Field not mapped to EPrints] AD - Golestan University of Medical Sciences, Iran [Field not mapped to EPrints] AD - Hamadan University of Medical Sciences, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: 554Leu/Phe, ARMS-PCR, Gene Polymorphism, Human, Ser128Arg, Soluble E-selectin, Visceral Leishmaniasis
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 18 Apr 2015 06:42
Last Modified: 25 Feb 2017 07:51
URI: http://eprints.goums.ac.ir/id/eprint/2302

Actions (login required)

View Item View Item