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In vitro inhibition of Helicobacter pylori urease with non and semi fermented Camellia sinensis

Shoae Hassani, A. and Ordouzadeh, N. and Ghaemi, A. and Amirmozafari, N. and Hamdi, K. and Nazari, R. (2009) In vitro inhibition of Helicobacter pylori urease with non and semi fermented Camellia sinensis. Indian Journal of Medical Microbiology, 27 (1). pp. 30-34. ISSN 02550857 (ISSN)

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Abstract

Purpose: Helicobacter pylori is the etiological agent in duodenal and peptic ulcers. The growing problem of antibiotic resistance by the organism demands the search for novel compounds, especially from natural sources. This study was conducted to evaluate the effect of Camellia sinensis extracts on the urease enzyme that is a major colonization factor for H. pylori. Methods: Minimum inhibitory concentrations of nonfermented and semifermented C. sinensis methanol: water extracts were assessed by broth dilution method. Examination of the urease function was performed by Mc Laren method, and urease production was detected on 12% SDS polyacrylamide gel electrophoresis from whole cell and membrane bound proteins. Results: Both extracts had inhibitory effects against H. pylori and urease production. At a concentration of 2.5 mg/ml of nonfermented extract and 3.5 mg/ml of semifermented extract the production of Ure A and Ure B subunits of the urease enzyme were inhibited completely. A concentration of 4 mg/ml of nonfermented and 5.5 mg/ml of semifermented extract were bactericidal for H. pylori. Conclusions: C. sinensis extracts, especially the nonfermented, could reduce H. pylori population and inhibit urease production at lower concentrations. The superior effect of nonfermented extract is due to its rich polyphenolic compounds and catechin contents.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Indian J. Med. Microbiol. [Field not mapped to EPrints] C2 - 19172056 [Field not mapped to EPrints] AD - Microbiology Department, Science and Research Branch of IAU, Tehran, Iran [Field not mapped to EPrints] AD - Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Microbiology Department, Iran Medical School, Tehran, Iran [Field not mapped to EPrints] AD - Basic Sciences Department, Lahijan Azad University, Guilan, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Camellia sinensis, Catechin, Helicobacter pylori, Urease, catechin, plant extract, polyphenol derivative, urease, water, antibiotic sensitivity, antimicrobial activity, article, bacterial colonization, broth dilution, fermentation inhibition, Helicobacter pylori, in vitro study, minimum inhibitory concentration, nonhuman, polyacrylamide gel electrophoresis, tea, whole cell, Anti-Bacterial Agents, Camellia sinensis, Enzyme Inhibitors, Helicobacter pylori, Microbial Sensitivity Tests, Microbial Viability, Plant Extracts, Urease
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 18 Apr 2015 04:56
Last Modified: 01 Mar 2017 10:21
URI: http://eprints.goums.ac.ir/id/eprint/2298

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