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Phage shock protein g, a novel ethanol-induced stress protein in salmonella typhimurium

Shoae Hassani, A. and Malekzadeh, F. and Amirmozafari, N. and Hamdi, K. and Ordouzadeh, N. and Ghaemi, A. (2009) Phage shock protein g, a novel ethanol-induced stress protein in salmonella typhimurium. Current Microbiology, 58 (3). pp. 239-244. ISSN 03438651 (ISSN)

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Abstract

Exposure to ethanol is a stress condition that Salmonella typhimurium often encounters during its life cycle. Food, beverage, drugs, and cosmetics have a long history of using alcohols to control pathogens. Ethanol is also commonly used for disinfecting medical instruments. This study was conducted to evaluate the ethanol stress variations on the protein profile, cell structure, and serologic features of S. typhimurium. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed the phage shock protein G (pspG), a new ethanol-induced stress protein in cells adapted to 10% ethanol. The result was confirmed by liquid chromatography-mass spectrometry. The maximum quantity of this 9.02-kDa protein was produced in 12.5% (v/v) of ethanol-treated cultures. Scanning electron microscopy has demonstrated new phenotypic characteristics in bacterial structure. The cells were unable to undergo binary fission. This phenomenon explains the tight attachment of bacteria in a colony. Overall, ethanol extreme stress induced expression of new proteins like PspG and repression of some other proteins in S. typhimurium. These induction and repression processes have inflicted dramatic changes on Salmonella behaviors. © 2008 Springer Science+Business Media, LLC.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Curr. Microbiol. [Field not mapped to EPrints] C2 - 19018590 [Field not mapped to EPrints] AD - Microbiology Department of Fars Science, Research Branch, Azad University, Fars, Iran [Field not mapped to EPrints] AD - Microbiology Department of Tehran Sciences, Research Branch, IAU, Tehran, Iran [Field not mapped to EPrints] AD - Microbiology Department, Iran University of Medical Sciences, Tehran, Iran [Field not mapped to EPrints] AD - Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: alcohol, bacterial protein, heat shock protein, phage shock protein G, unclassified drug, article, bacterium colony, cell structure, controlled study, liquid chromatography, mass spectrometry, nonhuman, polyacrylamide gel electrophoresis, priority journal, protein analysis, protein expression, protein induction, Salmonella typhimurium, scanning electron microscopy, serology, stress, Bacterial Proteins, Chromatography, Liquid, Ethanol, Heat-Shock Proteins, Mass Spectrometry, Molecular Weight, Salmonella typhimurium, Bacteria (microorganisms), Salmonella, Salmonella typhimurium
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 18 Apr 2015 03:41
Last Modified: 04 Mar 2017 04:58
URI: http://eprints.goums.ac.ir/id/eprint/2294

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