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Virulence increasing of salmonella typhimurium in Balb/c Mice after heat-stress induction of phage shock protein A

Hassani, A.S. and Amirmozafari, N. and Ghaemi, A. (2009) Virulence increasing of salmonella typhimurium in Balb/c Mice after heat-stress induction of phage shock protein A. Current Microbiology, 59 (4). pp. 446-450. ISSN 03438651 (ISSN)

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Abstract

View at Publisher| Export | Download | Add to List | More... Current Microbiology Volume 59, Issue 4, October 2009, Pages 446-450 Virulence increasing of salmonella typhimurium in Balb/c Mice after heat-stress induction of phage shock protein A (Article) Hassani, A.S.ab, Amirmozafari, N.c, Ghaemi, A.bd a Department of Microbiology, Fars Science and Research Branch of IAU, Shiraz, Fars, Iran b Young Researchers Club (YRC) of Science and Research Branch, Islamic Azad University, Tehran, Iran c Department of Microbiology, Iran University of Medical Sciences, Tehran, Iran View additional affiliations View references (39) Abstract Salmonella typhimurium is a potentially intracellular pathogen and is responsible for thousands of reported cases of acute gastroenteritis and diarrhea each year. Although many successful physiological and genetic approaches have been taken to conclude the key virulence determinants encoded by this organism, the total number of uncharacterized reading frames observed within the S. typhimurium genome suggests that many virulence factors remain to be discovered. This study was conducted to evaluate the role of heat induced phage shock protein A (PspA), in the pathogenicity of S. typhimurium. The stress proteins detected on sodium dodecyl sulfate-polyacrylamide gel electrophoresis were identified specifically by immunoblotting with polyclonal antibody against PspA. PspA was produced in response to heat stress at 45°C and it was over-expressed at 65°C. At this temperature, the stressed bacterial cells producing PspA were more virulent (16 folds greater) to female 6-8 week-old Balb/c mice. Correspondency between decrease in LD50 and increase in PspA production during heat stress and lower pathogenicity in non-producing cells that emerged during stress at 55°C represents PspA as an important virulence factor in heat stressed S. typhimurium. © 2009 Springer Science+Business Media, LLC.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Curr. Microbiol. [Field not mapped to EPrints] C2 - 19636618 [Field not mapped to EPrints] AD - Department of Microbiology, Fars Science and Research Branch of IAU, Shiraz, Fars, Iran [Field not mapped to EPrints] AD - Young Researchers Club (YRC) of Science and Research Branch, Islamic Azad University, Tehran, Iran [Field not mapped to EPrints] AD - Department of Microbiology, Iran University of Medical Sciences, Tehran, Iran [Field not mapped to EPrints] AD - Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: bacterial protein, heat shock protein, phage shock protein a, unclassified drug, virulence factor, animal experiment, animal model, article, bacterial virulence, controlled study, female, gene overexpression, heat stress, immunoblotting, mouse, nonhuman, nucleotide sequence, pathogenicity, polyacrylamide gel electrophoresis, priority journal, Salmonella typhimurium, Animals, Bacterial Proteins, Electrophoresis, Polyacrylamide Gel, Female, Heat-Shock Proteins, Hot Temperature, Lethal Dose 50, Mice, Mice, Inbred BALB C, Proteome, Salmonella typhimurium, Survival Analysis, Transcriptional Activation, Up-Regulation, Virulence, Bacteria (microorganisms), Mus, Salmonella typhimurium
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 18 Apr 2015 03:42
Last Modified: 04 Mar 2017 11:07
URI: http://eprints.goums.ac.ir/id/eprint/2256

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