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Effect of nitric oxide modulation on the basic and rate-dependent electrophysiological properties of AV-node in the isolated heart of rabbit: The role of adrenergic and cholinergic receptors

Khori, V. and Davarian, A. and Nayebpour, M. and Salaki, S. and Salehi, A. and Shirafkan, A.A. and Badaghabadi, F. and Pourabouk, M. and Alizadeh, A.M. and Changizi, S. (2010) Effect of nitric oxide modulation on the basic and rate-dependent electrophysiological properties of AV-node in the isolated heart of rabbit: The role of adrenergic and cholinergic receptors. Physiology and Pharmacology, 14 (1). pp. 12-22. ISSN 17350581 (ISSN)

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Abstract

Introduction: Recent studies showed that nitrergic system have specific modulatory effects on electrophysiological properties of atrioventricular (AV) node. The aim of this study was to determine the effects of nitric oxide (NO) on the electrophysiological properties of isolated rabbit AV node and to investigate the role of adrenergic and cholinergic receptors in the mechanism of its action. Methods: In our laboratory, an experimental model of isolated double-perfused AV-node of rabbits weighing 1.5-2 kg was used. Specific experimental protocols of recovery, Facilitation, Fatigue and Wenckbach were applied in both control and in the presence of the drug. A total number of 35 rabbits were divided randomly into the following groups (n=7): 1) L-Arg (NO donor) (250, 750 and 1000 μmol), 2) L- NAME, a NO synthesis inhibitor (25, 50 and 100 μmol), 3) L-Arg + L- NAME, 4) Nadolol (1 μmol), 5) Atropine (3 μmol). All data were shown as mean ± SE. The level of statistical significance was set at p<0.05. Results: Our results revealed the depressant effect of L-Arg on the basic and rate-dependent electrophysiological properties of AV-node. L- NAME did not deteriorate the effects of L-Arg on the basic and rate-dependent properties, nevertheless, at high concentration (100 μmol) it had a direct inhibitory effect on the AV-node. Nadolol and atropine could prevent the effects of NO on the basic nodal characteristics and the fatigue phenomenon, respectively. Conclusion: Nitergic system can affect basic and rate-dependent electrophysiological properties of the AV-node through adrenergic and cholinergic receptors.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - Persian [Field not mapped to EPrints] J2 - Physiol. Pharmacol. [Field not mapped to EPrints] AD - Golestan Cardiovascular Research Center, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Dept. Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran [Field not mapped to EPrints] AD - Cancer Research Center, Tehran University of Medical Sciences, Tehran, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Adrenergic, Cholinergic, Isolated AV-node, Nitric oxide, adrenergic receptor, arginine, atropine, cholinergic receptor, n(g) nitroarginine methyl ester, nadolol, nitric oxide, animal experiment, article, body weight, controlled study, facilitation, fatigue, heart atrioventricular node, heart electrophysiology, inhibition kinetics, isolated heart, modulation, nonhuman, rabbit, randomization
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 20 Apr 2015 07:59
Last Modified: 05 Apr 2017 10:04
URI: http://eprints.goums.ac.ir/id/eprint/2223

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