Golestan University of Medical Sciences Repository

The role of CA1 α-adrenoceptor on scopolamine induced memory impairment in male rats

Azami, N.S. and Piri, M. and Jahanshahi, M. and Oryan, S. and Babapour, V. and Zarrindast, M.R. (2010) The role of CA1 α-adrenoceptor on scopolamine induced memory impairment in male rats. Physiology and Pharmacology, 14 (1). pp. 66-77. ISSN 17350581 (ISSN)

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Abstract

Introduction: Similarities in the memory impairment between Alzheimer patients and scopolamine treated animals have been reported. In the present study, the possible role of α-adrenergic receptors of the dorsal hippocampus on scopolamine state-dependent memory in adult male Wistar rats was evaluated. Methods: The animals were bilaterally implanted with chronic cannulae in the CA1 regions of the dorsal hippocampus, trained in a step-through type inhibitory avoidance task, and tested 24 h after training to measure step-through latency. Results: Post-training intra-CA1 administration of scopolamine (0.5 and 2μg/rat) dose-dependently reduced the step-through latency, showing an amnestic response. Amnesia produced by post-training scopolamine (2 μg/rat) was reversed by pre-test administration of the scopolamine (0.5 and 2 μg/rat) that is due to a state-dependent effect. Pre-test intra-CA1 injection of α1-adrenoceptor agonist, phenylephrine (0.25, 0.5 μg/rat) in the dose range that we used, could not affect memory impairment induced by post-training injection of scopolamine (2 μg/rat). However intra-CA1 pretest injection of α2-adrenoceptor agonist, clonidine (0.5 μg/rat) improved post-training scopolamine (2 μg/rat) intra-CA1 injection induced retrieval impairment. Furthermore, pre-test intra-CA1 microinjection of phenylephrine (0.25 and 0.5 μg/rat) or clonidine (0.25 and 0.5 μg/rat) with an ineffective dose of scopolamine (0.25 μg/rat), synergistically improved memory performance impaired by post-training scopolamine (2 μg/rat). Our results also showed that, pre-test injection of α1-receptor antagonist prazosin (1, 2 μg/rat) or α2-receptors antagonist yohimbine (1, 2 μg/rat) before effective dose of scopolamine (2 μg/rat) prevented the improvement of memory by pre-test scopolamine. Conclusion: These results suggest that α1- and α2-adrenergic receptors of the dorsal hippocampal CA1 region may play an important role in scopolamine-induced amnesia and scopolamine state-dependent memory.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - Persian [Field not mapped to EPrints] J2 - Physiol. Pharmacol. [Field not mapped to EPrints] AD - Dept. Biology, Faculty of Basic Sciences, Islamic Azad University, Gorgan, Iran [Field not mapped to EPrints] AD - Dept. Biology, Faculty of Basic Sciences, Islamic Azad University, Ardabil, Iran [Field not mapped to EPrints] AD - Neuroscience Research Centre, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Dept. Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran [Field not mapped to EPrints] AD - Dept. Physiology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran [Field not mapped to EPrints] AD - Dept. Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: α-adrenergic receptors, Dorsal hippocampus, Inhibitory avoidance response, Scopolamine, State-dependent learning, alpha 1 adrenergic receptor, alpha 2 adrenergic receptor, clonidine, phenylephrine, prazosin, scopolamine, yohimbine, amnesia, article, cannula, hippocampus, human, latent period, male, memory disorder, nonhuman, rat, state dependent learning
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 20 Apr 2015 05:50
Last Modified: 05 Apr 2017 09:16
URI: http://eprints.goums.ac.ir/id/eprint/2221

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