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Identification of Leishmania species isolated from human Cutaneous leishmaniasis in gonbad-e-Qabus city using a PCR method during 2006-2007

Mesgarian, F. and Rahbarian, N. and Rad, M.M. and Hajaran, H. and Shahbaz, F. and Mesgarian, Z. and Taghipour, N. (2010) Identification of Leishmania species isolated from human Cutaneous leishmaniasis in gonbad-e-Qabus city using a PCR method during 2006-2007. Tehran University Medical Journal, 68 (4). pp. 250-256. ISSN 16831764 (ISSN)

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Abstract

Background: Cutaneous Leishmaniasis is endemic in plenty of Iranian provinces. This study aimed to determine the epidemiological status of the cutaneous Leishmaniasis outbreak, isolation and identification of the parasite using a PCR method in burden rural areas of Gonbad-e-Qabus County, north Iran. Methods: Data was collected on the prevalence of scars and ulcers over a period of three months among 6990 inhabitants of five villages around Gonbad-e-Qabus county, north Iran, during 2006-2007. Cultured promastigotes were identified using PCR technique. ITS1 and ITS2 of Non Coding Transcribed region at ribosomal DNA of 46 Leishmania isolates were amplified and the PCR products were separated by electrophoresis in 1.5% agarose gel (200 mA, 140 V), visualized by staining with ethidium bromide, and photographed. To confirm the PCR findings, six Leishmanis isolates were injected individually into two BALB/c mice. Results: Among 6990 inhabitants of the five villages, 62.9% had scars and 0.5% had active lesions. The most highly infected age group was 0-10 years and nobody was infected in individuals more than fifty years of age. Individuals 11 to 20 years of age were the most highly infected age group. The results showed that from 46 isolates, all (100%) were L. major in comparison to reference strains and all of them could produce ulcer at the base tail of BALB/c mice, 4-12 weeks after inoculation. Conclusions: According to this study, cutaneous Leishmaniasis due to Leishmania major is endemic in Gonbad-e-Qabus county, north Iran. The results were confirmed by active lesions induced in BALB/c mice.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Tehran Uni. Med. J. [Field not mapped to EPrints] AD - Gonbad-e Qabus Health Center, Golestan University of Medical Sciences, Golestan, Iran [Field not mapped to EPrints] AD - Cellular and Molecular Biology Research Cente, Shahid Behesthi University, M.C., Tehran, Iran [Field not mapped to EPrints] AD - Department of Medical Parasitology and Mycology, Medicine Faculty, Shahid Behesthi University, M.C., Tehran, Iran [Field not mapped to EPrints] AD - Skin Research Center, Shahid Behesthi University, M.C., Tehran, Iran [Field not mapped to EPrints] AD - Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Cutaneous leishmaniasis, Golestan, Gonbad-e-Qabus, Leishmania major, PCR, ribosome DNA, adolescent, adult, agar gel electrophoresis, article, child, controlled study, epidemic, gene amplification, human, infant, Iran, Leishmania major, major clinical study, mouse, nonhuman, polymerase chain reaction, preschool child, prevalence, promastigote, scar, school child, skin leishmaniasis, species identification, ulcer
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 20 Apr 2015 03:40
Last Modified: 20 Jun 2015 07:45
URI: http://eprints.goums.ac.ir/id/eprint/2205

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