Kia, E.B. and Rahimi, H. and Sharbatkhori, M. and Talebi, A. and Fasihi Harandi, M. and Mirhendi, H. (2010) Genotype identification of human cystic echinococcosis in Isfahan, central Iran. Parasitology Research, 107 (3). pp. 757-760. ISSN 09320113 (ISSN)
Full text not available from this repository.Abstract
Echinococcosis/hydatidosis is one of the most important zoonotic diseases commonly found in different regions of Iran with a major economic and public health importance. In the current study, Echinococcus granulosus isolates were collected from hospitalized patients in Isfahan, central Iran. The genotypes of 30 samples were determined by polymerase chain reaction amplification of internal transcribed spacer-1 region of ribosomal DNA, followed by restriction fragment length polymorphism (RFLP) with two restriction enzymes namely AluI and MspI. As expected, each isolate yielded an approximately 1-kbp DNA fragment on the electrophoresis gel. According to RFLP results for both enzymes, all isolates had an equal pattern indicating the G1 genotype. Our findings confirmed that G1 is the dominant genotype of cystic echinococcosis in human in central Iran, with predilection to different organs including liver, lung, and brain, and warrants the importance of sheep dog cycle in public health. © 2010 Springer-Verlag.
| Item Type: | Article |
|---|---|
| Additional Information: | Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Parasitol. Res. [Field not mapped to EPrints] C2 - 20549235 [Field not mapped to EPrints] AD - Department of Medical Parasitology and Mycology, School of Public Health and National Institute of Health Research, Tehran University of Medical Sciences, Tehran, Iran [Field not mapped to EPrints] AD - Al-Zahra Hospital, Isfahan University of Medical Sciences, Isfahan, Iran [Field not mapped to EPrints] AD - Department of Medical Parasitology and Mycology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Department of Medical Parasitology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints] |
| Uncontrolled Keywords: | DNA fragment, internal transcribed spacer 1, ribosome DNA, adult, article, brain echinococcosis, controlled study, echinococcosis, Echinococcus granulosus, female, genotype, human, Iran, liver hydatid cyst, lung hydatid cyst, major clinical study, male, nonhuman, parasite isolation, polymerase chain reaction, priority journal, restriction fragment length polymorphism, Animals, Deoxyribonuclease HpaII, Deoxyribonucleases, Type II Site-Specific, DNA, Helminth, DNA, Ribosomal Spacer, Echinococcosis, Echinococcus granulosus, Genotype, Humans, Iran, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, Canis familiaris, Echinococcus granulosus, Ovis aries |
| Subjects: | مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus |
| Divisions: | معاونت تحقیقات و فناوری |
| Depositing User: | GOUMS |
| Date Deposited: | 20 Apr 2015 03:39 |
| Last Modified: | 17 Jan 2017 11:35 |
| URI: | http://eprints.goums.ac.ir/id/eprint/2196 |
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