Golestan University of Medical Sciences Repository

Protective role of Urtica dioica L. (Urticaceae) extract on hepatocytes morphometric changes in STZ diabetic Wistar rats

Golalipour, M.J. and Ghafari, S. and Afshar, M. (2010) Protective role of Urtica dioica L. (Urticaceae) extract on hepatocytes morphometric changes in STZ diabetic Wistar rats. Turkish Journal of Gastroenterology, 21 (3). pp. 262-269. ISSN 13004948 (ISSN)

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Abstract

Background/aims: The present investigation was carried out to evaluate the protective effect of the hydroalcoholic extract of Urtica dioica leaves on the quantitative morphometric changes in the liver of streptozotocin-induced diabetic rats. Methods: Thirty male Wistar rats were divided into control (G1), diabetic (G2), diabetic + Urtica dioica (G3) groups. The control group received only sham injections of intraperitoneal saline; the diabetic group received intraperitoneal saline for 5 days followed by streptozotocin (80 mg/kg) on the 6th day; and the diabetic + Urtica dioica group received 100 mg/kg Urtica dioica intraperitoneal (7) injections for 5 days and streptozotocin injection on the 6th day. After five weeks, the animals were sacrificed and whole livers removed. Liver specimens were used for quantitative morphometric analysis after hematoxylin and eosin staining. All data were statistically analyzed by one-way ANOVA and expressed as the mean with standard error of means. Results: In the G3 (diabetic + Urtica diocia) group, the mean surface area of hepatocytes in the periportal zone (Z1) was greater than in G2 (diabetic) and G1 (control) groups, but this difference was not significant. No alteration was observed in the surface area of hepatocytes in the perivenous zone (Z3) in the diabetic+Urtica dioica (G3) group compared to the diabetic (G2) group. The mean nuclear area of hepatocytes of the rats in the diabetic+Urtica dioica (G3) group was higher in Z1 and lower in Z3 than that of rats in the diabetic (G2) group. The mean diameter of hepatocyte nuclei in the diabetic + Urtica dioica (G3) group was lower than that of diabetic (G2) and control (G1) groups in both Z1 and Z3. Conclusions: This study revealed that the administration of extract of Urtica dioica leaves before induction of diabetic with streptozotocin has a protective effect on the morphometric alterations of hepatocytes in the periportal and perivenous zones of the liver lobule in rats.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Turk. J. Gastroenterol. [Field not mapped to EPrints] C2 - 20931430 [Field not mapped to EPrints] AD - Gorgan University of Medical Sciences, Department of Embryology and Histology, P.O. Box 49175-553, Gorgan, Iran [Field not mapped to EPrints] AD - Department of Medical Sciences, Birjand University of Embryology and Histology, Birjand, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Morphometry, Periportal/perivenous zone, Rat liver, Streptozotocin diabetes, Urtica dioica extract, glucose, liver protective agent, sodium chloride, streptozocin, Urtica dioica extract, animal cell, animal experiment, animal tissue, article, cell nucleus, cell protection, cell surface, cellular distribution, controlled study, glucose blood level, glucose tolerance test, liver cell, liver histology, male, morphometrics, newborn, nonhuman, nucleolus, plant leaf, quantitative analysis, rat, streptozocin diabetes, Wistar rat, Animals, Diabetes Mellitus, Experimental, Hepatocytes, Male, Phytotherapy, Plant Extracts, Rats, Rats, Wistar, Streptozocin, Urticaceae
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 20 Apr 2015 06:04
Last Modified: 23 May 2015 06:21
URI: http://eprints.goums.ac.ir/id/eprint/2189

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