Golestan University of Medical Sciences Repository

Spa diversity among MRSA and MSSA strains of Staphylococcus aureus in north of Iran

Shakeri, F. and Shojai, A. and Golalipour, M. and Alang, S.R. and Vaez, H. and Ghaemi, E.A. (2010) Spa diversity among MRSA and MSSA strains of Staphylococcus aureus in north of Iran. International Journal of Microbiology. ISSN 1687918X (ISSN)

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Abstract

Protein A of Staphylococcus aureus is a pathogenic factor whose encoding gene, spa, shows a variation in length in different strains. In this study the spa gene variation in S. aureus isolated from healthy carriers and patients was studied, We also compared this variation among MRSA with MSSA strains. 208 strains of Staphylococcus aureus which we were isolated from Gorgan, north of Iran were studied, 121 cases from patients and 87 cases from healthy carriers, 59 out of them were MRSA and 149 MSSA. Samples DNA were extracted and amplified by specific primer of spa gene. In 4 (3.8%) strains of them no spa gene was detected, and 10.6% had a dual band (1200 and 1400 bp). In strains with one band, the length of spa gene differed from 1150 to 1500 bp. The most prevalent length was 1350-1400 bp (37%). The frequencies of short spa bands (1150-1200 bp) in patients strains were significantly higher. In 4 (3.8%) strains of them no spa gene was detected, and 10.6% had a dual band (1200 and 1400 bp). In strains with one band, the length of spa gene differed from 1150 to 1500 bp. The most prevalent length was 1350-1400 bp (37%). The frequencies of short spa bands (1150-1200 bp) in patients strains were significantly higher. The spa gene length of 1350-1400 bp in MSSA was more than in MRSA strains (P< .05). The average length of spa in isolated strains from urinary tract infections was more than others. It is concluded that the length of spa gene depends either on resistance to Methicillin or the source of S. aureus isolation. Copyright © 2010 Fatemeh Shakeri et al.

Item Type: Article
Additional Information: Unmapped bibliographic data: C7 - 351397 [EPrints field already has value set] LA - English [Field not mapped to EPrints] J2 - Int. J. Microbiol. [Field not mapped to EPrints] AD - Islamic Azad University of Lahijan, Lahijan, Iran [Field not mapped to EPrints] AD - Basic Sciences Faculty, Golestan University, Gorgan, Iran [Field not mapped to EPrints] AD - Golestan University of Medical Sciences, Gorgon 4934174515, Iran [Field not mapped to EPrints] AD - Microbiology Department, Infectious Disease Research Center, Gorgan, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: methicillin resistant Staphylococcus aureus, Staphylococcus aureus
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 14 Apr 2015 09:43
Last Modified: 17 Jan 2017 11:35
URI: http://eprints.goums.ac.ir/id/eprint/2166

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