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Protection of mice by a λ-based therapeutic vaccine against cancer associated with human papillomavirus type 16

Ghaemi, A. and Soleimanjahi, H. and Gill, P. and Hassan, Z.M. and Razeghi, S. and Fazeli, M. and Razavinikoo, S.M.H. (2011) Protection of mice by a λ-based therapeutic vaccine against cancer associated with human papillomavirus type 16. Intervirology, 54 (3). pp. 105-112. ISSN 03005526 (ISSN)

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Abstract

Objective: Human papillomavirus (HPV) oncoproteins (i.e. E6 and E7) are constitutively expressed in cervical cancer cells. The proteins are ideal targets to be used for developing therapeutic vaccines against existing HPV-associated carcinomas. To date, whole bacteriophage ('phage')-λ particles, rather than purified 'naked' DNA, have been described as highly efficient delivery vehicles for a DNA vaccine. Methods: In this study, a safe and efficient λ-based therapeutic cancer vaccine, recombinant λ-ZAP E7 phage, was developed by inserting a HPV16 E7 gene into the Lambda ZAP® cytomegalovirus vector. λ-ZAP E7 phages were employed to immunize mice against the E7-expressing murine tumor cell line (TC-1), which is used as a tumor model in an H-2b murine system. Results: The tumor-bearing mice indicated a significant inhibition of tumor growth after 3 injections of 2 � 1012 particles of recombinant phages. Released lactate dehydrogenase, interferon-γ and granzyme B from spleen cells and lymphocyte proliferation of spleen cells, which all demonstrate the enhancement of cell-mediated immunity, suggested the phages could be a potent gene delivery system in animal models. Conclusion: Our results suggest the recombinant phages can be used as effective biological tools for inducing E7-specific protective immune responses. Hence, the study introduces a possible therapeutic strategy against cervical cancer and other HPV-related neoplasia. Copyright © 2010 S. Karger AG, Basel.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Intervirology [Field not mapped to EPrints] C2 - 20956855 [Field not mapped to EPrints] AD - Faculty of Medicine, Golestan University of Medical Sciences and Health Care, Gorgan, Iran [Field not mapped to EPrints] AD - Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, PO Box 14115-111, Tehran, Iran [Field not mapped to EPrints] AD - Department of Immunology, Faculty of Medical Sciences, Iran [Field not mapped to EPrints] AD - Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Iran [Field not mapped to EPrints] AD - Shefa Neuroscience Research Centre, Tehran, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: C57BL/6 mice, Cervical cancer, Gene therapy, Human papillomavirus E7, Phage-based nanoparticles, complementary DNA, DNA vaccine, gamma interferon, granzyme B, lactate dehydrogenase, nanoparticle, plasmid DNA, Wart virus vaccine, animal cell, animal experiment, article, bacteriophage, C57BL 6 mouse, cellular immunity, controlled study, Escherichia coli, gene delivery system, gene therapy, growth inhibition, Human papillomavirus type 16, lymphocyte proliferation, mouse, nonhuman, priority journal, spleen cell, tumor growth, uterine cervix cancer, virus infection, Animals, Bacteriophage lambda, Cancer Vaccines, Disease Models, Animal, Female, Genetic Vectors, Immunotherapy, Mice, Mice, Inbred C57BL, Neoplasms, Papillomavirus E7 Proteins, Papillomavirus Vaccines, Treatment Outcome, Animalia, Cytomegalovirus, Human papillomavirus, Human papillomavirus type 16, Murinae, Mus
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 18 Apr 2015 05:32
Last Modified: 14 May 2015 03:13
URI: http://eprints.goums.ac.ir/id/eprint/2130

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