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Frequency-dependent anti arrhythmic effects of crataegus monogyna on the extracellular field potential recordings in the rabbit atrioventricular node, an experimental model of AF

Khori, V. and Azadbakht, M. and Nayebpour, M. and Jamshidi, A.H. and Pourabouk, M. and Alizadeh, A.M. and Badaghabadi, F. and Changizi, S. and Rajaei, M. (2011) Frequency-dependent anti arrhythmic effects of crataegus monogyna on the extracellular field potential recordings in the rabbit atrioventricular node, an experimental model of AF. Physiology and Pharmacology, 15 (1). pp. 36-46. ISSN 17350581 (ISSN)

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Abstract

Introduction: Despite extensive studies that have been performed on the effects of Crataegus monogyna in cardiovascular diseases, only few investigations have addressed the antiarrhythmic properties of this plant. Aims of the present study were: 1) To determine the protective role of methanolic extract of C. monogyna on the rate-dependent model and the concealed conduction of the AV node. 2) To explore the role of Na+-K+ ATPase in the protective role of C. monogyna Methods: Male New Zealand rabbits (1.5-2kg) were used in all experiments. Stimulation protocols were used to measure basic and rate-dependent AV nodal properties (recovery, atrial fibrilation and zone of concealment) in two groups (N=14). In the first group, all the stimulation protocols were performed before and after the administration of different concentrations of C. monogyna extract (n=7), while in the second group (n=7), all stimulation protocols were carried out in the presence of ouabaine (0.05 μM) and the plant extract. Results: Basic and rate-dependent properties of the AV node were inhibited after the addition of the extract of C. monogyna to Kerebs Henselite solution. At the maximum concentration of C. monogyna (30 mg/l), WBCL cycle length was significantly increased from 156.5±3.4 to 173±5.8 ms and the nodal functional refractory period was prolonged from 164.4±4.1 to 182.7±3.8 ms (P<0.05). Significant decreases of ventricular rhythm were recorded in both selective concentrations of the plant extract. The depressant electrophysiological effect of C. monogyna on the AV node was not abolished by ouabaine, a selective inhibitor of Na+-K+ ATPase enzyme. Conclusion: The results showed a potential anti-arrhythmic and protective effect for C. monogyna. The effect of the plant extract in increasing nodal refractory period and widening of the concealment zone might be the major mechanisms involved. The protective role of C. monogyna was not related to the Na+-K+ ATPase activity.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Physiol. Pharmacol. [Field not mapped to EPrints] AD - Golestan Cardiovascular research center, Golestan university of medical sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Faculty of pharmacy, Sari university of medical sciences, Sari, Iran [Field not mapped to EPrints] AD - Dept. pharmacology, Faculty of pharmacy, Tehran university of medical sciences, Tehran, Iran [Field not mapped to EPrints] AD - Dept. Pharmacognosy, Iranian institute of medicinal plant, Minestry of health, Tehrsan, Iran [Field not mapped to EPrints] AD - Cancer research center, Tehran university of medical sinces, Tehran, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Hydroalcoholic extract of C. monogyna, Rate-dependent properties, Zone of concealment, adenosine triphosphatase (potassium sodium), antiarrhythmic agent, Crataegus extract, Crataegus monogyna extract, ouabain, unclassified drug, animal experiment, animal model, article, concentration response, Crataegus, crataegus monogyna, electrophysiology, enzyme activity, heart atrioventricular node, heart atrium fibrillation, heart protection, heart rhythm, heart ventricle conduction, male, nonhuman, rabbit, refractory period
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 20 Apr 2015 08:42
Last Modified: 16 May 2015 07:52
URI: http://eprints.goums.ac.ir/id/eprint/2120

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