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Anti fungal activity of alcoholic extract of Peganum harmala seeds

Diba, K. and Shoar, M.G. and Shabatkhori, M. and Khorshivand, Z. (2011) Anti fungal activity of alcoholic extract of Peganum harmala seeds. Journal of Medicinal Plant Research, 5 (23). pp. 5550-5554. ISSN 19960875 (ISSN)

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In our study, it was aimed to determine antifungal activity of Peganum harmala on medically important yeasts and molds in vitro. Fungal suspensions of Candida spp and aspergillus spp isolated from the clinical specimens were treated with serial dilutions of P. harmala alcoholic extract in Sabouraud Broth. The titers included: 1/20, 1/40, 1/80, 1/160, 1/320, 1/640, and incubated for 24 h at 30°C. Anti fungal activity of the extract was determined as MIC and MFC. For the determination of MFC, a small volume of above serial dilutions was cultured on SGA 4% medium. After 24 to 72 h of incubation at 30°C, the cultures were looked for fungal growth. The highest and lowest inhibitory effects of P. harmala extract were determined on C. glabrata (MIC: 0.312 mg/ml) and C. albicans (MIC: 1.25 mg/ml), respectively. The MFC for Candida species was differed from 0.62 to 2.5 mg/ml. The highest fungicidal effect was seen on C. glabrata and C. tropicalis (MFC: 0.62 and 0.125 mg/ml, respectively) and the lowest was for C. albicans (MFC: 2.5 mg/ml). The treatment of Aspergillus suspensions with Perganum extract showed a decreasing of growth and sporolation without definite inhibition. The alcoholic extract of P. harmala showed a fungicidal activity on opportunistic yeasts, Candida spp, and a decreasing sporolation for aspergillus most importamt species. © 2011 Academic Journals.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - J. Med. Plant Res. [Field not mapped to EPrints] AD - Department of Medical Parasitology and Mycology, School of Medicine, Urmia University of Medical Sciences, Uromia, Iran [Field not mapped to EPrints] AD - Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran [Field not mapped to EPrints] AD - Infectious Diseases Research Center, Department of Medical Parasitology and Mycology, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Fungi, Growth inhibitory, Peganum harmala, Peganum harmala extract, agar medium, antifungal activity, article, Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, broth dilution, Candida albicans, Candida dubliniensis, Candida glabrata, Candida parapsilosis, Candida tropicalis, concentration response, controlled study, drug determination, drug screening, fungus growth, fungus isolation, growth inhibition, in vitro study, incubation time, minimum inhibitory concentration, nonhuman, plant seed, Aspergillus, Candida, Candida albicans, Candida glabrata, Candida tropicalis, Fungi, Peganum harmala
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 19 Apr 2015 04:09
Last Modified: 13 May 2015 19:43
URI: http://eprints.goums.ac.ir/id/eprint/2087

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