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Glycated hemoglobin measurements from dried blood spots: Reliability and relation to results obtained from whole blood samples

Tabatabaei-Malazy, O. and Heshmat, R. and Omidfar, K. and Pasalar, P. and Delavari, A. and Keshtkar, A. and Larijani, B. (2011) Glycated hemoglobin measurements from dried blood spots: Reliability and relation to results obtained from whole blood samples. Iranian Journal of Diabetes and Lipid Disorders, 10. pp. 1-6. ISSN 17267544 (ISSN)

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Background: Main objective was to measure glycated hemoglobin (HbA1c) in dried blood spots on paper filter and in whole blood samples in diabetic patients to evaluate relationship between two methods and their respective reliability. Methods: The 20�10 μl of venous blood samples of 33 diabetics were blotted onto the filter paper allowed to dry at room temperature and then stored at 25°C and 4°C. HbA1c was measured via the Turbidimetric Inhibition Immunoassay Technique. The relation was evaluated with correlation and linear regression tests using STATA software and SPSS. Agreement between the results obtained from the dried blood spots and others was evaluated using the Bland and Altman. The pitman's permutation test was also employed to compare the difference in variance. Results: A high positive correlation was detected between whole blood samples and dried blood spots stored at 4°C (r2 =0.90) and at 25°C (r2 = 0.95). The Bland and Altman graphs, as well as the Pitman tests, showed statistically significant differences in variability between the values obtained from whole blood samples and those derived from dried spots stored at 4°C (p=0.05) or 25°C (p=0.004). Conclusion: HbA1c measurements from dried blood spots on the filter paper yielded reliable results. That the Hitachi autoanalyzer is available in most countries renders this assay less costly than the High Performance Liquid Chromatography Method (HPLC). In addition, the filter paper method for Immuno-turbidimetric estimations of HbA1c at different temperatures is reliable and may be particularly useful in outpatient diabetes clinic.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Iran. J. Diabetes Lipid Disord. [Field not mapped to EPrints] AD - Endocrinology and Metabolism Research Center, Tehran University of Medical Science, Shariati Hospital, North Kargar Street, 14114 Tehran, Iran [Field not mapped to EPrints] AD - Digestive Disease Research Center, Tehran University of Medical Sciences, Tehran, Iran [Field not mapped to EPrints] AD - Gastrology and Hepatology Research Center, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Diabetes, Glycated hemoglobin, Paper filter, Ried blood spot, hemoglobin A1c, adult, aged, article, blood analysis, blood filter, blood sampling, clinical article, controlled study, data analysis software, diabetic patient, diagnostic test accuracy study, dried blood spot, female, human, immunoassay, interrater reliability, laboratory test, male, non insulin dependent diabetes mellitus, room temperature, sensitivity and specificity, somatic hypermutation, Turbidimetric Inhibition Immunoassay, turbidimetry, venous blood
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 18 Apr 2015 06:29
Last Modified: 16 May 2015 15:37
URI: http://eprints.goums.ac.ir/id/eprint/2085

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