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Effect of morphine based CPP on the hippocampal astrocytes of male wistar rats

Shaabani, R. and Jahanshahi, M. and Nowrouzian, M. and Sadeghi, Y. and Azami, N.S. (2011) Effect of morphine based CPP on the hippocampal astrocytes of male wistar rats. Asian Journal of Cell Biology, 6 (3). pp. 89-96. ISSN 18140068 (ISSN)

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Abstract

Opioid abuse is an increasing problem worldwide and the reinforcing effects of opioid analgesics make them susceptible to diversion and illicit use and abuse. As morphological changes in different parts of the brain including the hippocampus and dentate gyrus that have Plasticity is looking for a lot of drug injection in human and animal studies has been viewed. Also other evidences have shown that Astrocytes actively participate in synaptic plasticity. Therefore the aim of this study was obtain of Conditioning Place Preference (CPP) on cell density (Astrocytes number) of rat hippocampal formation. In present study, 48 male Wistar rat weighted average 220-250 g were used. For behavioral tests, rats divided into eight groups and experimental groups daily (three days) received morphine at different doses (2.5, 5, 7.5 mg kg-1) by subcutaneous injection and sham groups, received saline dose (1 mL kg-1) and then CPP test in them were investigated. 48 h after behavioral testing animals were decapitated under chloroform anesthesia and their brains fixed and after tissue processing, slices stained with GFAP immunohistochemistery techniques. Present results showed the most dose responses of morphine were observed in 7.5 mg kg-1. It also revealed that don't exist any differences between the number of astrocytes in CAj and CAg in control group and control-saline, but this difference between control and the other groups was significant statistically (p<0.05). We concluded that the phenomenon of morphine based conditioned place preference can cause a significant increase in the number of astrocytes in experimental groups with compared to controls. © 2011 Academic Journals Inc.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Asian J. Cell Biol. [Field not mapped to EPrints] AD - Department of Anatomy, Shahid Beheshti University of Medical Sciences, Tehran, Iran [Field not mapped to EPrints] AD - Neuroscience Research Center, Department of Anatomy, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Department of Biology, Gorgan Branch, Islamic Azad University, Gorgan, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Astrocytes, Conditioning place preference, Hippocampus rats, Immunohistochemistery, Morphine
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 15 Apr 2015 09:11
Last Modified: 23 May 2015 09:15
URI: http://eprints.goums.ac.ir/id/eprint/2062

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