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Effect of pre-treatment with oxytocin on cardiac enzymes in regional ischemia-reperfusion injury induced in the rat heart

Alizadeh, A.M. and Faghihi, M. and Khori, V. and Mohsenikia, M. (2012) Effect of pre-treatment with oxytocin on cardiac enzymes in regional ischemia-reperfusion injury induced in the rat heart. Physiology and Pharmacology, 15 (4). pp. 572-582. ISSN 17350581 (ISSN)

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Abstract

Introduction: Cardiac preconditioning represents the most potent and consistently reproducible method of rescuing heart tissue from undergoing irreversible ischemic damage. The aim of the present study was to evaluate oxytocin (OT) induced cardioprotection and its signaling pathways on lactate dehydrogenase (LDH) and creatine kinase-MB isoenzyme (CK-MB) in the anesthetized rats. Methods: Eighty-four rats were divided into fourteen groups. Animal's hearts were subjected to 25 min ischemia and 2 h reperfusion (I/R). Oxytocin (0.03 μg/kg) was used 25 min prior to ischemia. Atosiban, an OT receptor antagonist (1.5 μg/kg), 5-hydroxydecanoic acid, an inhibitor of mitochondrial ATP-dependent potassium channel (10 mg/kg), atractyloside, an opener of mitochondrial permeability transition pores (5 mg/kg), chelytraine, a protein kinase C inhibitor (5 mg/kg) and N-acetylcysteine, a reactive oxygen species scavenger (200 mg/kg) were used 10 min prior to OT administration. Then, LDH and CK-MB levels in plasma were measured. Results: OT administration significantly decreased CK-MB and LDH levels compared to I/R group. Administration of atosiban, 5-hydroxydecanoic, atractyloside, chelytraine and N-acetylcysteine abolished the cardiac preconditioning effect of OT. Conclusion: The present study demonstrates that oxytocin cardioprotective effects are complex and its signaling pathways may mediate through mKATP channels, mPTP, PKC activation and increase ROS.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - Persian [Field not mapped to EPrints] J2 - Physiol. Pharmacol. [Field not mapped to EPrints] AD - Cancer Research Center, Tehran University of Medical Science, Tehran, Iran [Field not mapped to EPrints] AD - Dept. physiology, School of Medicine, Tehran University of Medical Science, Tehran, Iran [Field not mapped to EPrints] AD - Dept. Pharmacology, Golestan Cardiovascular Research Center, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Islamic Azad University, Tehran Medical Beranch, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: 5-hydroxydecanoic, Atractyloside, Chelytraine, Ischemia-reperfusion, N-acetylcysteine, Oxytocin, 5 hydroxydecanoic acid, acetylcysteine, atosiban, atractyloside, chelytraine, creatine kinase MB, heart enzyme, lactate dehydrogenase, oxytocin, protein kinase C inhibitor, reactive oxygen metabolite, unclassified drug, animal experiment, animal model, Article, heart protection, nonhuman, rat, reperfusion injury, signal transduction
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 20 Apr 2015 04:01
Last Modified: 25 Oct 2016 07:55
URI: http://eprints.goums.ac.ir/id/eprint/2061

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