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Detection of extended spectrum B-lactamases in urinary isolates of Klebsiella pneumoniae in relation to Bla SHV, Bla TEM and Bla CTX-M gene carriage

Eftekhar, F. and Rastegar, M. and Golalipoor, M. and Mansoursamaei, N. (2012) Detection of extended spectrum B-lactamases in urinary isolates of Klebsiella pneumoniae in relation to Bla SHV, Bla TEM and Bla CTX-M gene carriage. Iranian Journal of Public Health, 41 (3). pp. 127-132. ISSN 03044556 (ISSN)

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Abstract

Background: Resistance to contemporary broad-spectrum β-lactam antibiotics mediated by extended-spectrum β-lactamases (ESBLs) is increasing worldwide. Klebsiella pneumoniae, an important cause of nosocomial and community acquired urinary tract infections has rapidly become the most common ESBL producing organism. We examined ESBL production in urinary isolates of K. pneumoniae in relation to the presence of bla SHV, bla TEM and bla CTX-M genes. Methods: Antibiotic resistance of 51 clinical isolates of K. pneumoniae was determined to amoxicillin, amikacin, ceftazidime, cefotaxime, cefteriaxon, ceftizoxime, gentamicin, ciprofloxacin and nitrofurantoin by disc diffusion. Minimum inhibitory concentrations were also measured for ceftazidime, cefotaxime, cefteriaxon, ceftizoxime and ciprofloxacin. ESBL production was detected by the double disc synergy test and finally, presence of the bla SHV, bla TEM and bla CTX-M genes were shown using specific primers and PCR. Results: Disc diffusion results showed that 96.08 % of the isolates were resistant to amoxicillin followed by 78.43 % resistance to nitrofurantoin, 49.02 % to amikacin and ceftazidime, 41.17 % to ceftriaxone, 37.25% resistance to cefotaxime and ceftizoxime, and 29.42 % to gentamicin and ciprofloxacin. Both resistant and intermediately resistant organisms were resistant in MIC determinations. Twenty two isolates (43.14%) carried bla SHV, 18 (35.29%) had bla TEM and 16 (31.37%) harbored bla CTX-M genes. ESBL production was present in 14 isolates (27.45 %) of which, 3 did not harbor any of the 3 genes. Among the non- ESBL producers, 9 lacked all 3 genes and 2 carried them all. Conclusion: No relation was found between gene presence and ESBL expression.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Iran. J. Public Health [Field not mapped to EPrints] AD - Dept. of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, Evin, Tehran, Iran [Field not mapped to EPrints] AD - Dept. of Basic Sciences, Faculty of Science, Golestan University, Gorgan, Iran [Field not mapped to EPrints] AD - Dept. of Human Genetics, Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Bbla CTX-M, bla SHV, bla TEM, Extended spectrum β-lactamases, Klebsiella pneumoniae, Urinary infection
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 18 Apr 2015 06:58
Last Modified: 11 May 2015 14:31
URI: http://eprints.goums.ac.ir/id/eprint/2030

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