Golestan University of Medical Sciences Repository

Effect of Vitamin E on Oocytes Apoptosis in nicotine-treated Mice

Asadi, E. and Jahanshahi, M. and Golalipour, M.J. (2012) Effect of Vitamin E on Oocytes Apoptosis in nicotine-treated Mice. Iranian Journal of Basic Medical Sciences, 15 (3). pp. 880-884. ISSN 20083866 (ISSN)

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Abstract

Objective(s): Cigarette and nicotine enhances embryogenesis, fertility, pregnancy loss and ultrastructure alterations of oocyte. This study was performed to determine the effect of daily supplementation of vitamin E on oocytes apoptosis in nicotine-treated mice. Materials and Methods: In this experimental study, 24 NMARI adult female mice were randomly allocated into four experimental groups. For 30 days, animals in control group (C) were received saline through subcutaneous injection, group I received vitamin E (60 mg/kg/day orally), group II received nicotine (5 mg/kg/day, subcutaneous) and animals of group III received nicotine with vitamin E (60 mg/kg/day orally). After 30 days, the animals were superovulated with PSMG (10 Units) and HCG (10 Units). Next day animals were sacrificed and oocytes were flushed. Collected oocytes were examined through TUNEL assay for the determination of apoptosis through the use of fluorescent microscope. Results: The number of retrieved oocytes was 139, 148, 97 and 127 in control, experimental group I, II and III, respectively. Nicotine treatment increased apoptosis in oocytes up to 13.4% whereas oocytes apoptosis was 3.6% in controls. Supplementation with vitamin E in nicotine-treated mice reduced the oocytes apoptosis to 5.5%. Conclusion: This study showed that nicotine exposure (5 mg/kg/day for 30 days) can increase apoptosis in oocytes, and supplementation with vitamin E (60 mg/kg/day orally) can reduce the oocytes apoptosis in nicotine-treated mice.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Iran. J. Basic Med. Sci. [Field not mapped to EPrints] AD - Department of Anatomical Sciences, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Gorgan Congenital Malformation Research Centre, Department of Anatomical Sciences, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Antioxidant, Cell death, Nicotine, Oocytes, TUNEL assay, alpha tocopherol, chorionic gonadotropin, nicotine, seric gonadotropin, sodium chloride, animal cell, animal experiment, apoptosis, article, controlled study, female, fluorescence microscopy, mouse, nick end labeling, nonhuman, oocyte, oocyte retrieval, superovulation, vitamin supplementation
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 18 Apr 2015 05:41
Last Modified: 11 May 2015 07:29
URI: http://eprints.goums.ac.ir/id/eprint/2021

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