Golestan University of Medical Sciences Repository

Mutations in the S gene region of hepatitis B virus genotype D in Golestan Province-Iran

Moradi, A. and Zhand, S. and Ghaemi, A. and Javid, N. and Tabarraei, A. (2012) Mutations in the S gene region of hepatitis B virus genotype D in Golestan Province-Iran. Virus Genes, 44 (3). pp. 382-387. ISSN 09208569 (ISSN)

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Abstract

Mutations of HBsAg especially within the "a" determinant could alter the antigenicity of the protein causing failure of HBsAg neutralization and escaping from the host's immune system, resulting in active viral replication and liver disease. This project aimed to investigate mutation in the S gene region of HBV infected patients in Golestan Province-Iran. HBV-DNA extractions from plasma and PCR of 100 patients were performed. Direct sequencing and alignment of S gene were applied using reference sequence from Gene Bank database. All isolates were belonged to genotype D, subgenotype D1, subtype ayw2. Overall 92 point mutations occurred. Of them, 40 (43.47%) were missense and 52 (56.52%) were silent. Mutations were detected in 95 cases (95%). Five of 40 mutations (12.5%) occurred in "a" determinant and 13 (32.5%), 17 (42.5%), and 2 (5%) were seen in antigenic epitope regions of B cell, CD 4 + and CTL, respectively. Frame shift mutations were seen in 22 cases (22%). 14% of mutations occurred at Major Hydrophilic Region(MHR) area which P120T/S and R122K/T substitutions were the most frequent ones (4%). Mutation in G145R of the S gene was observed in one case. A large number of MHR mutants are in association with failure of HBsAg detection, vaccine, and immunotherapy escape. This study showed "a" determinant S gene mutations in HBV infected people with HBsAg positivity in Golestan Province-Iran. The rate of mutation in our study was 95%. Collectively, the results of this project exhibited that most of mutations were clustered in CD 4 + antigenic epitopes. © Springer Science+Business Media, LLC 2012.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Virus Genes [Field not mapped to EPrints] C2 - 22274739 [Field not mapped to EPrints] AD - Infectious Diseases Research Centre, Department of Microbiology, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Golestan, Hepatitis B virus, Iran, Mutation, S gene, arginine, epitope, glycine, hepatitis B surface antigen, hepatitis B vaccine, immunoglobulin, lysine, proline, serine, threonine, virus DNA, adult, amino acid substitution, antigen detection, article, B lymphocyte, CD4+ T lymphocyte, cytotoxic T lymphocyte, DNA extraction, DNA sequence, drug treatment failure, female, frameshift mutation, gene mutation, genotype, hepatitis B, Hepatitis B virus, human, immunotherapy, Iran, major clinical study, male, missense mutation, mutational analysis, nonhuman, nucleotide sequence, point mutation, polymerase chain reaction, priority journal, S gene, sequence alignment, virus gene, virus isolation, Adult, Cluster Analysis, DNA, Viral, Epitopes, B-Lymphocyte, Epitopes, T-Lymphocyte, Female, Frameshift Mutation, Genotype, Hepatitis B, Hepatitis B Surface Antigens, Hepatitis B virus, Humans, Iran, Male, Mutation, Missense, Plasma, Point Mutation, Polymerase Chain Reaction, Sequence Alignment, Sequence Analysis, DNA, Hepatitis B virus
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 20 Apr 2015 08:08
Last Modified: 12 May 2015 09:39
URI: http://eprints.goums.ac.ir/id/eprint/1998

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