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Impact of fumonisin B1 on the production of inflammatory cytokines by gastric and colon cell lines

Mahmoodi, M. and Alizadeh, A.M. and Sohanaki, H. and Rezaei, N. and Amini-Najafi, F. and Khosravi, A.R. and Hosseini, S.K. and Safari, Z. and Hydarnasab, D. and Khori, V. (2012) Impact of fumonisin B1 on the production of inflammatory cytokines by gastric and colon cell lines. Iranian Journal of Allergy, Asthma and Immunology, 11 (2). pp. 165-173. ISSN 17351502 (ISSN)

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Abstract

Fumonisins, a family of mycotoxins, are mainly toxic and carcinogenic. The present study was carried out to evaluate fumonisin B1 (FB1) effects on the production of inflammatory cytokines by gastric and colon cell lines. The study was performed on two cell lines under in vitro condition, including gastric epithelial cell line (AGS) and human colon adenocarcinoma cell line (SW742). Lipopolysaccharide (LPS) was used for inflammatory cytokine induction. The culture medium was supplemented with 4.5-72 mg/l of FB1 for 72 h before cell induction. The supernatants were harvested 24 h after the induction and measured for cytokines by using enzyme-linked immunosorbent assay. FB1 induced a dose-dependent increase in the production of tumor necrosis factor-/ and interleukin-10 in both AGS and SW742 cell lines. This increase was statistically significant with concentration of FB1 between 9 and 72 mg/l (P < 0.05). FB1 also induced a dosedependent decrease in interleukin-8 production. This decrease was seen in both cell lines and showed a statistical significance with FB1 concentration (P < 0.05). The results show that FB1 increases inflammatory cytokines production by various gastric and intestinal cells. This effect in the long run can possibly be the basis for the occurrence or development of inflammation and subsequent atrophy in the above-mentioned tissues. Copyright© 2012, Iranian Journal of Allergy, Asthma and Immunology. All rights reserved.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Iran. J. Allergy Asthma Immunol. [Field not mapped to EPrints] C2 - 22761190 [Field not mapped to EPrints] AD - Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran [Field not mapped to EPrints] AD - Cancer Research Center of Cancer Institute, Tehran University of Medical Sciences, P.O: 1419733141, Tehran, Iran [Field not mapped to EPrints] AD - Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran [Field not mapped to EPrints] AD - Department of Immunology, Molecular Immunology Research Center, Tehran University of Medical Sciences, Tehran, Iran [Field not mapped to EPrints] AD - Department of Pathology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran [Field not mapped to EPrints] AD - Department of Medical Parastiology, Mycology, School of Medicine, Tehran University, Tehran, Iran [Field not mapped to EPrints] AD - Tissue Bank Research and Preparation Center, Tehran University of Medical Sciences, Tehran, Iran [Field not mapped to EPrints] AD - Golestan Cardiovascular Research Center, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Colon, Fumonisin B1, Gastric, IL-1β, IL-8, TNF-α, fumonisin B1, interleukin 1beta, interleukin 8, lipopolysaccharide, tumor necrosis factor alpha, article, cancer cell culture, carcinoma cell, cell culture, colon adenocarcinoma, cytotoxicity, enzyme linked immunosorbent assay, epithelium cell, human, human cell, in vitro study, stomach epithelium, Cell Line, Tumor, Colon, Culture Media, Conditioned, Cytokines, Dose-Response Relationship, Drug, Enzyme-Linked Immunosorbent Assay, Fumonisins, Humans, Inflammation Mediators, Interleukin-1beta, Interleukin-8, Stomach, Time Factors, Tumor Necrosis Factor-alpha
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 18 Apr 2015 05:39
Last Modified: 25 Oct 2016 07:57
URI: http://eprints.goums.ac.ir/id/eprint/1997

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