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Sequence analysis of the second internal Transcribed spacer (ITS2) region of rDNA for species identification of trichostrongylus nematodes isolated from domestic livestock in Iran

Ghasemikhah, R. and Sharbatkhori, M. and Mobedi, I. and Kia, E. and Fasihi Harandi, M. and Mirhendi, H. (2012) Sequence analysis of the second internal Transcribed spacer (ITS2) region of rDNA for species identification of trichostrongylus nematodes isolated from domestic livestock in Iran. Iranian Journal of Parasitology, 7 (2). pp. 40-46. ISSN 17357020 (ISSN)

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Abstract

Background: Infectivity of herbivores with Trichostrongylus nematodes is widespread in many countries, having a major economic impact on breeding, survivability, and productivity of domestic livestock. This study was carried out on Trichostrongylus species isolated from domestic livestock in order to develop an easy-to-perform method for species identification. Methods: Trichostrongylus isolates were collected from sheep, goat, cattle, and buffaloes in Khuzestan Province, southwest Iran. Primary species identification was carried out based on morphological characterization of male worms. PCR amplification of ITS2-rDNA region was performed on genomic DNA and the products were sequenced. Phylogenetic analysis of the nucleotide sequence data was conducted employing Bayesian Inference approach. Consequently, a restriction fragment length polymorphism (RFLP) profile was designed to differentiate Trichostrongylus species. Results: A consensus sequence of 238 nucleotides was deposited in the GenBank for Iranian isolates of Trichostrongylus species including T. colubriformis, T. capricola, T. probolurus and T. vitrinus. The designated RFLP using restriction enzyme TasI could readily differentiate among species having different ITS2 sequence. The molecular analysis was in concordance with morphological findings. Conclusion: Phylogenetic analysis indicated a close relationship among the sequences obtained in this study and reference sequence of relevant species. ITS2-RFLP with TasI is recommended for molecular differentiation of common Trichostrongylus species.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Iran. J. Parasitol. [Field not mapped to EPrints] AD - Vali-e-asr Hospital, Arak University of Medical Sciences, Arak, Iran [Field not mapped to EPrints] AD - Department of Medical Parasitology and Mycology, School of Public Health, National Institute of Health Research, Tehran University of Medical Sciences, Tehran, Iran [Field not mapped to EPrints] AD - Laboratory Science Research Center, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Infectious Diseases Research Center, Department of Medical Parasitology and Mycology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Department of Parasitology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Iran, ITS2, Livestock, Trichostrongylus, genomic DNA, internal transcribed spacer 2, ribosome DNA, article, buffalo, cattle, domestic animal, gene amplification, goat, Iran, livestock, molecular genetics, nematode, nonhuman, nucleotide sequence, parasite isolation, phylogeny, polymerase chain reaction, restriction fragment length polymorphism, sequence analysis, sheep, species differentiation, species identification, Trichostrongylus, Trichostrongylus capricola, Trichostrongylus colubriformis, trichostrongylus vitrinus
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 18 Apr 2015 06:44
Last Modified: 11 May 2015 15:21
URI: http://eprints.goums.ac.ir/id/eprint/1995

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