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Oxytocin protects cardiomyocytes from apoptosis induced by ischemia-reperfusion in rat heart: Role of mitochondrial ATP-dependent potassium channel and permeability transition pore

Alizadeh, A.M. and Faghihi, M. and Khori, V. and Sohanaki, H. and Pourkhalili, K. and Mohammadghasemi, F. and Mohsenikia, M. (2012) Oxytocin protects cardiomyocytes from apoptosis induced by ischemia-reperfusion in rat heart: Role of mitochondrial ATP-dependent potassium channel and permeability transition pore. Peptides, 36 (1). pp. 71-77. ISSN 01969781 (ISSN)

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Abstract

The current study examines the protective effect of oxytocin (OT) on cardiomyocyte apoptosis modulated by mitochondrial ATP-dependent potassium (mitoKATP) channel and permeability transition pore (mPTP) in the preconditioned myocardium of anesthetized rats. Eighty rats were equally divided into eight groups. The hearts of all animals except for the sham group were subjected to 25 min ischemia and 120 min reperfusion. Oxytocin, 5-hydroxydeconoate (5-HD), a specific inhibitor of the mitoKATP channel, and atractyloside (ATRC), an mPTP opener, were used prior to ischemia. Hemodynamic parameters were recorded throughout the experiment. Evaluations were made by infarct size, plasma lactate dehydrogenase level (LDH), transmission electron microscopy (TEM) and immunohistochemistry studies. OT prevented mean arterial pressure drop during early phase of ischemia and reperfusion. Treatment with OT before IR induction normalizes cardiomyocytes both in light microscopy and TEM observations. In addition, OT significantly reduced TUNEL- and increased Bcl-2-labeled positive cell number relative to IR (p < 0.05). However, 5HD or ATRC inhibited the protective effects of OT on cardiomyocytes damaged by IR (p < 0.05). Ultrastructural changes including extensive myofibril loss, sarcolemmal disruption and mitochondrial swelling due to amorphous dens bodies indicate necrosis induction in 5HD and ATRC as well as in IR groups. Restoration of immunohistochemistry parameters and protection against IR-induced ultrastructural changes confirm OT cardioprotective effects via mitoKATP channel and mPTP modulation in apoptosis induced by ischemia-reperfusion. © 2012 Elsevier Inc.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Peptides [Field not mapped to EPrints] C2 - 22504012 [Field not mapped to EPrints] AD - Cancer Research Center, Tehran University of Medical Science, Tehran, Iran [Field not mapped to EPrints] AD - Department of Physiology, School of Medicine, Tehran University of Medical Science, Tehran, Iran [Field not mapped to EPrints] AD - Department of Pharmacology, Golestan Cardiovascular Research Center, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Department of Physiology, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran [Field not mapped to EPrints] AD - Cellular and Molecular Research Center, Faculty of Medicine, Gilan University of Medical Sciences, P.O. Box: 3477, Rasht, Iran [Field not mapped to EPrints] AD - Islamic Azad University, Tehran Medical Branch, Tehran, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: 5HD, Apoptosis, Atractyloside, Bcl-2, Cardioprotection, Oxytocin, 5 hydroxydeconoate, adenosine triphosphatase, enzyme inhibitor, lactate dehydrogenase, mitochondrial adenosine triphosphatase dependent potassium channel, mitochondrial permeability transition pore, oxytocin, protein bcl 2, unclassified drug, animal experiment, animal model, apoptosis, article, biochemistry, cell count, drug mechanism, heart infarction, heart infarction size, heart mitochondrion, heart muscle cell, heart protection, hemodynamic parameters, immunohistochemistry, lactate dehydrogenase blood level, male, mean arterial pressure, microscopy, muscle fibril, nick end labeling, nonhuman, priority journal, protein function, rat, reperfusion injury, sarcolemma, transmission electron microscopy, Animals, Apoptosis, Hemodynamics, L-Lactate Dehydrogenase, Male, Microscopy, Electron, Transmission, Mitochondria, Heart, Mitochondrial Membrane Transport Proteins, Myocardial Reperfusion Injury, Myocytes, Cardiac, Oxytocin, Potassium Channels, Rats, Rats, Sprague-Dawley, Animalia, Rattus
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 20 Apr 2015 03:45
Last Modified: 25 Oct 2016 07:57
URI: http://eprints.goums.ac.ir/id/eprint/1990

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