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Modulation of the hepatocyte rough endoplasmic reticulum single chloride channel by nucleotide-Mg 2+ interaction

Ashrafpour, M. and Babaei, J.F. and Saghiri, R. and Sepehri, H. and Sharifi, H. (2012) Modulation of the hepatocyte rough endoplasmic reticulum single chloride channel by nucleotide-Mg 2+ interaction. Pflugers Archiv European Journal of Physiology, 464 (2). pp. 175-182. ISSN 00316768 (ISSN)

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Abstract

The effect of nucleotides on single chloride channels derived from rat hepatocyte rough endoplasmic reticulum vesicles incorporated into bilayer lipid membrane was investigated. The single chloride channel currents were measured in 200/50 mmol/l KCl cis/trans solutions. Adding 2.5 mM adenosine triphosphate (ATP) and adenosine diphosphate (ADP) did not influence channel activity. However, MgATP addition inhibited the chloride channels by decreasing the channel open probability (Po) and current amplitude, whereas mixture of Mg 2+ and ADP activated the chloride channel by increasing the Po and unitary current amplitude. According to the results, there is a novel regulation mechanism for rough endoplasmic reticulum (RER) Cl - channel activity by intracellular MgATP and mixture of Mg 2+ and ADP that would result in significant inhibition by MgATP and activation by mixture of Mg 2+ and ADP. These modulatory effects of nucleotide-Mg 2+ complexes on chloride channels may be dependent on their chemical structure configuration. It seems that Mg-nucleotide-ion channel interactions are involved to produce a regulatory response for RER chloride channels. © Springer-Verlag 2012.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Pflug. Arch. Eur. J. Physiol. [Field not mapped to EPrints] C2 - 22684478 [Field not mapped to EPrints] AD - Cellular and Molecular Research Center, Department of Physiology and Pharmacology, Babol University of Medical Sciences, Babol, Iran [Field not mapped to EPrints] AD - Neuroscience Research Center, Department of Physiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran [Field not mapped to EPrints] AD - Department of Biochemistry, Pasteur Institute of Iran, Tehran, Iran [Field not mapped to EPrints] AD - Neuroscience Research Center, Department of Physiology, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Bilayer lipid membrane, Chloride channels, MgATP-MgADP, Rough endoplasmic reticulum, Single channel properties, 4,4' diisothiocyanatostilbene 2,2' disulfonic acid, adenosine diphosphate, adenosine triphosphate, adenosine triphosphate magnesium, chloride channel, magnesium ion, animal cell, article, cell level, cell membrane potential, channel gating, chloride current, concentration response, controlled study, lipid bilayer, liver cell, male, molecular interaction, nonhuman, pharmacological blocking, pharmacological stimulation, priority journal, rat, regulatory mechanism, rough endoplasmic reticulum, structure activity relation, 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid, Adenosine Diphosphate, Adenosine Triphosphate, Animals, Chloride Channels, Endoplasmic Reticulum, Rough, Hepatocytes, Ion Channel Gating, Male, Patch-Clamp Techniques, Rats, Rats, Wistar
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 20 Apr 2015 05:14
Last Modified: 11 May 2015 07:37
URI: http://eprints.goums.ac.ir/id/eprint/1980

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