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Genotyping Echinococcus granulosus from dogs from Western Iran

Parsa, F. and Fasihi Harandi, M. and Rostami, S. and Sharbatkhori, M. (2012) Genotyping Echinococcus granulosus from dogs from Western Iran. Experimental Parasitology, 132 (2). pp. 308-312. ISSN 00144894 (ISSN)

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Abstract

Cystic echinococcosis is a zoonotic infection caused by the dog tapeworm, Echinococcus granulosus. In the present study, adults of E. granulosus (n = 20) were collected from 71 dogs from Western Iran and were genetically characterized using DNA sequencing of the partial mitochondrial cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase 1 (nad1). Consensus sequences were obtained for cox1 (366) and nad1 (471) genes. Phylogenetic analysis of concatenated nad1 and cox1 nucleotide sequence data was performed using Bayesian Inference approach. Overall, the dog isolates indicated nine different sequences in cox1 and seven in nad1 genes. Three genotypes (G1 [75%], G2 [10%] and G3 [15%]) were identified from the isolates. The G2 sequences indicated 100% homology with reference G2 sequence in both cox1 (Genbank accession number M84662) and nad1 (AJ237633) genes. G3 sequences showed 100% homology with G3 reference sequence in nad1 (AJ237633), but displayed two different cox1 profiles, each having 99% homology with reference G3 sequence (M84663). In the phylogenetic tree all of the isolates were grouped into a distinct cluster corresponding to the G1-G3 complex with relevant reference sequences. The presence of G1 genotype (sheep strain) of E. granulosus sensu stricto as dominant genotype in dogs is emphasized. To the best of our knowledge, this study established the first record of E. granulosus sensu stricto, G2 genotype in Iran. © 2012 Elsevier Inc.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Exp. Parasitol. [Field not mapped to EPrints] C2 - 22884512 [Field not mapped to EPrints] AD - Department of Laboratory Sciences, Faculty of Para-Medicine, Islamic Azad University, Borujerd Branch, Borujerd, Iran [Field not mapped to EPrints] AD - Zoonoses Research Center, Department of Parasitology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran [Field not mapped to EPrints] AD - Laboratory Science Research Center, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Department of Parasitology and Mycology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Dog, Echinococcus granulosus, G2 genotype, Iran, Mitochondrial genome, cytochrome c oxidase, article, cestode life cycle stage, controlled study, cox1 gene, DNA sequence, dog, Echinococcus granulosus, gene, gene cluster, gene identification, genotype, haplotype, Iran, nad1 gene, nonhuman, nucleotide sequence, phylogeny, priority journal, sequence homology, Animals, DNA, Helminth, Dog Diseases, Dogs, Echinococcosis, Echinococcus granulosus, Electron Transport Complex IV, Female, Genotype, Genotyping Techniques, Iran, Male, NAD, Canis familiaris, Echinococcus granulosus, Ovis aries
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 18 Apr 2015 04:13
Last Modified: 12 May 2015 10:09
URI: http://eprints.goums.ac.ir/id/eprint/1964

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