Golestan University of Medical Sciences Repository

A plausible anti-apoptotic role of up-regulated OCT4B1 in bladder tumors

Asadzadeh, J. and Asadi, M.H. and Shakhssalim, N. and Rafiee, M.-R. and Kalhor, H.R. and Tavallaei, M. and Mowla, S.J. (2012) A plausible anti-apoptotic role of up-regulated OCT4B1 in bladder tumors. Urology Journal, 9 (3). pp. 574-580. ISSN 17351308 (ISSN)

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Abstract

Purpose: To investigate and compare the expression of OCT4B1 between tumor and non-tumor bladder tissues. Materials and Methods: We investigated the expression of OCT4B1 in 30 tumor and non-tumor surgical specimens of the bladder, using the TaqMan real-time polymerase chain reaction approach and by carefully designing primers and probes specific for the amplification of the variant. Results: Most tumor and non-tumor samples of the bladder showed OCT4B1 expression, but its expression level was significantly higher in the tumors (P <.002). Moreover, the up-regu-lation of OCT4B1 was more significant in high-grade tumors compared to the low-grade ones (P <.05). We have also employed the RNA interference strategy to evaluate the functional role of OCT4B1 in a bladder cancer cell line, 5637. Suppression of OCT4B1 caused some changes in cell cycle distribution, and significantly elevated the rate of apoptosis in the cells. Conclusion: Our findings suggest that OCT4B1 plays a potential role in tumor initiation and/or progression of the bladder cancer. Additionally, OCT4B1 can be regarded as a new tumor marker for detection, classification, and treatment of the bladder cancer. However, more ex-perimental studies are needed to replicate our findings.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Urol. J. [Field not mapped to EPrints] C2 - 22903480 [Field not mapped to EPrints] AD - Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran [Field not mapped to EPrints] AD - Department of Biotechnology, Research Institute of Environmental Sciences, International Center for Science, High Technology and Environmental Sciences, Kerman, Iran [Field not mapped to EPrints] AD - Urology and Nephrology Research Center, Shahid Labbafinejad Medical Center, Shahid Beheshti University of Medical sciences, Tehran, Iran [Field not mapped to EPrints] AD - Nanomedicine and Tissue Engineering Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran [Field not mapped to EPrints] AD - Omics Research Center, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Human Genetics Research Center, Baqiyatallah Medical Sciences University, Tehran, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Apoptosis, Cancer stem cells, Neoplasm invasiveness, Prognosis, Urinary bladder neoplasms, octamer transcription factor 4, tumor marker, apoptosis, article, bladder tumor, cell cycle, female, gene expression, gene expression regulation, genetics, human, male, metabolism, reverse transcription polymerase chain reaction, RNA interference, tumor cell line, upregulation, Apoptosis, Cell Cycle, Cell Line, Tumor, Female, Gene Expression, Gene Expression Regulation, Neoplastic, Humans, Male, Octamer Transcription Factor-3, Reverse Transcriptase Polymerase Chain Reaction, RNA Interference, Tumor Markers, Biological, Up-Regulation, Urinary Bladder Neoplasms
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 20 Apr 2015 05:22
Last Modified: 11 May 2015 07:33
URI: http://eprints.goums.ac.ir/id/eprint/1955

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