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Drug-related mutational patterns in hepatitis B virus (HBV) reverse transcriptase proteins from Iranian treatment-Naïve chronic HBV patients

Mahabadi, M. and Norouzi, M. and Alavian, S.M. and Samimirad, K. and Azad, T.M. and Saberfar, E. and Mahmoodi, M. and Ramezani, F. and Karimzadeh, H. and Malekzadeh, R. and Montazeri, G. and Nejatizadeh, A. and Ziee, M. and Abedi, F. and Ataei, B. and Yaran, M. and Sayad, B. and Somi, M.H. and Sarizadeh, G. and Sanei-Moghaddam, I. and Mansour-Ghanaei, F. and Rafatpanah, H. and Pourhosseingholi, M.A. and Keyvani, H. and Kalantari, E. and Saberfiroozi, M. and Judaki, M.A. and Ghamari, S. and Daram, M. and Fazeli, Z. and Goodarzi, Z. and Khedive, A. and Moradi, A. and Jazayeri, S.M. (2013) Drug-related mutational patterns in hepatitis B virus (HBV) reverse transcriptase proteins from Iranian treatment-Naïve chronic HBV patients. Hepatitis Monthly, 13 (1). ISSN 1735143X (ISSN)

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Abstract

Background: Immunomodulators and Nucleotide analogues have been used globally for the dealing of chronic hepatitis B virus (HBV) infection. However, the development of drug resistance is a major limitation to their long-term effectiveness. Objectives: The aim of this study was to characterize the hepatitis B virus reverse transcriptase (RT) protein variations among Iranian chronic HBV carriers who did not receive any antiviral treatments. Materials and Methods: Hepatitis B virus partial RT genes from 325 chronic in active carrier patients were amplified and directly sequenced. Nucleotide/amino acid substitutions were identified compared to the sequences obtained from the database. Results: All strains belonging to genotype D.365 amino-acid substitutions were found. Mutations related to lamivudine, adefovir, telbivudine, and entecavir occurred in (YMDD) 4% (n = 13), (SVQ) 17.23% (n = 56), (M204I/V + L180M) 2.45% (n = 8) and (M204I) 2.76% (n = 9) of patients, respectively. Conclusions: RT mutants do occur naturally and could be found in HBV carriers who have never received antiviral therapy. However, mutations related to drug resistance in Iranian treatment-naïve chronic HBV patients were found to be higher than other studies published formerly. Chronic HBV patients should be monitored closely prior the commencement of therapy to achieve the best regimen option. © 2013, KOWSAR Corp.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Hepatitis Mon. [Field not mapped to EPrints] AD - Hepatitis B Molecular Laboratory, Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR, Iran [Field not mapped to EPrints] AD - Middle East Liver Diseases Center (MELD Centers), Tehran, IR, Iran [Field not mapped to EPrints] AD - Hepatitis C Molecular Laboratory, Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR, Iran [Field not mapped to EPrints] AD - The research and Development Department of Bayerpaul Vaccine And Pharmaceutical Company, Tehran, IR, Iran [Field not mapped to EPrints] AD - Department of Epidemiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR, Iran [Field not mapped to EPrints] AD - Digestive Disease Research Center, Tehran University of Medical Sciences, Tehran, IR, Iran [Field not mapped to EPrints] AD - Research Center for Molecular Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, IR, Iran [Field not mapped to EPrints] AD - Hepatitis Research center, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, IR, Iran [Field not mapped to EPrints] AD - Department of Infectious Disease, Mashhad University of Medical Sciences, Mashhad, IR, Iran [Field not mapped to EPrints] AD - Gastrointestinal and Liver Diseases Research Center, Guilan University of Medical Sciences, Rasht, IR, Iran [Field not mapped to EPrints] AD - Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, IR, Iran [Field not mapped to EPrints] AD - Kermanshah Liver Diseases and Hepatitis Research Center, Kermanshah, IR, Iran [Field not mapped to EPrints] AD - Liver and Gastrointestinal Disease Research Center, Tabriz University of Medical Sciences, Tabriz, IR, Iran [Field not mapped to EPrints] AD - Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, IR, Iran [Field not mapped to EPrints] AD - Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IR, Iran [Field not mapped to EPrints] AD - Department of Virology, School of Medicine, Tehran University of Medical Sciences, Tehran, IR, Iran [Field not mapped to EPrints] AD - Gholhack Medical Laboratory, Tehran, IR, Iran [Field not mapped to EPrints] AD - Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, IR, Iran [Field not mapped to EPrints] AD - Department of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, IR, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Drug-resistance, Hepatitis B virus, Iran, Therapy, adefovir, entecavir, hepatitis B surface antibody, hepatitis B surface antigen, lamivudine, RNA directed DNA polymerase, telbivudine, adult, amino acid substitution, antiviral resistance, article, cross-sectional study, DNA sequence, enzyme linked immunosorbent assay, female, gene mutation, genotype, hepatitis B, Hepatitis B virus, human, Iran, major clinical study, male, mutation rate, mutational analysis, nucleotide sequence, phylogeny, polymerase chain reaction
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 18 Apr 2015 04:47
Last Modified: 15 May 2015 09:37
URI: http://eprints.goums.ac.ir/id/eprint/1908

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