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Immunomodulatory effects of human umbilical cord wharton's Jelly-Derived mesenchymal stem cells on differentiation, maturation and endocytosis of monocyte-derived dendritic cells

Saeidi, M. and Masoud, A. and Shakiba, Y. and Hadjati, J. and Bonab, M.M. and Nicknam, M.H. and Latifpour, M. and Nikbin, B. (2013) Immunomodulatory effects of human umbilical cord wharton's Jelly-Derived mesenchymal stem cells on differentiation, maturation and endocytosis of monocyte-derived dendritic cells. Iranian Journal of Allergy, Asthma and Immunology, 12 (1). pp. 37-49. ISSN 17351502 (ISSN)

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Abstract

The Wharton's jelly of the umbilical cord is believed to be a source of mesenchymal stem cells (MSCs) which can be therapeutically applied in degenerative diseases. In this study, we investigated the immunomodulatory effect of umbilical cord derivedmesenchymal stem cells (UC-MSCs) and bone marrow-derived-mesenchymal stem cells (BM-MSCs) on differentiation, maturation, and endocytosis of monocyte-derived dendritic cells in a transwell culture system under laboratory conditions. Monocytes were differentiated into immature dendritic cells (iDCs) in the presence of GM-CSF and IL-4 for 6 days and then differentiated into mature dendritic cells (mDCs) in the presence of TNF-for 2 days. In every stage of differentiation, immature and mature dendritic cells were separately cocultured with UC-MSCs and BM-MSCs. The findings showed that UC-MSCs and BM-MSCs inhibited strongly differentiation and maturation of dendritic cells at higher dilution ratios (1:1). The BM-MSCs and UC-MSCs showed more inhibitory effect on CD1a, CD83, CD86 expression, and dendritic cell endocytic activity, respectively. On the other hand, these cells severely up-regulated CD14 marker expression. We concluded that UC-MSCs and BM-MSCs could inhibit differentiation, maturation and endocytosis in monocyte-derived DCs through the secreted factors and free of any cellcell contacts under laboratory conditions. As DCs are believed to be the main antigen presenting cells for naive T cells in triggering immune responses, it would be logical that their inhibitory effect on differentiation, maturation and function can decrease or modulate immune and inflammatory responses. Copyright © Spring 2013, Iran J Allergy Asthma Immunol. All rights reserved.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Iran. J. Allergy Asthma Immunol. [Field not mapped to EPrints] C2 - 23454777 [Field not mapped to EPrints] AD - Department of Immunology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran [Field not mapped to EPrints] AD - Molecular Immunology Research Center and Department of Immunology, Tehran University of Medical Sciences, Tehran, Iran [Field not mapped to EPrints] AD - Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Bone marrow, Dendritic cell, Endocytosis, Mesenchymal stem cells, Wharton's jelly, 5' nucleotidase, CD14 antigen, CD34 antigen, CD83 antigen, CD86 antigen, endoglin, t6 antigen, Thy 1 antigen, article, bone marrow derived mesenchymal stem cell, cell differentiation, cell maturation, cell viability, controlled study, dendritic cell, endocytosis, flow cytometry, human, human cell, immunomodulation, in vitro study, mesenchymal stem cell, monocyte, osteocyte, peripheral blood mononuclear cell, protein expression, umbilical cord derived mesenchymal stem cell, upregulation, Wharton jelly, Adipocytes, Antigens, CD, Bone Marrow Cells, Cell Differentiation, Coculture Techniques, Dendritic Cells, Diffusion Chambers, Culture, Endocytosis, Gene Expression Regulation, Granulocyte-Macrophage Colony-Stimulating Factor, Humans, Immunomodulation, Interleukin-4, Mesenchymal Stromal Cells, Monocytes, Osteocytes, Primary Cell Culture, Tumor Necrosis Factor-alpha, Umbilical Cord, Wharton Jelly
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 18 Apr 2015 05:40
Last Modified: 10 May 2015 15:15
URI: http://eprints.goums.ac.ir/id/eprint/1903

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